Tryptic Soy Agar with Lecithin and Tween 80

Item No.  NCM0011A

$92.50
$849.00
$1,725.00
$4,826.00
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Tryptic Soy Agar with Lecithin and Tween 80 is used for the isolation of microorganisms from surfaces sanitized with quaternary ammonium compounds and is not intended for use in the diagnosis of disease or other conditions in humans.

In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing “preservative carryover”. Tryptic Soy Agar with Lecithin and Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).

Specifications
Analyte Non-selective
Brand NEOGEN Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 15.0 g/L
Enzymatic Digest of Soybean Meal 5.0 g/L
Sodium Chloride 5.0 g/L
Lecithin 0.7 g/L
Tween 80 5.0 g/L
Agar 20.5 g/L
Grams per Liter 45.70
Liters per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 600.00
Preparation
  1. Suspend 19.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.
Previous Item Number Product was previously known as Acumedia Product: 7163 Tryptic Soy Agar with Lecithin & Tween 80; and LabM Product: LAB189 Microbial Content Test Agar (MCTA)

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