Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar

Item No.  NCM0052A

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Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans.

TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic. Infections have been associated with ingestion of contaminated water and consumption of contaminated shellfish or seafood. Vibrio spp. are natural inhabitants of seawater.

According to ISO 21872-2, pre-incubation in Alkaline Saline Peptone Water (ASPW) (NCM0175) is required.

Analyte Vibrio spp.
Brand NEOGEN Culture Media
Formula Liter
Yeast Extract 5.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 5.0 g/L
Sodium Citrate 10.0 g/L
Sodium Thiosulfate 10.0 g/L
Bile Salts 8.0 g/L
Sucrose 20.0 g/L
Sodium Chloride 10.0 g/L
Ferric Citrate 1.0 g/L
Bromothymol Blue 0.04 g/L
Thymol Blue 0.04 g/L
Agar *14.0 g/L
Grams per Liter 88.00
Liters per 500g 6.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Suspend 88 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Previous Item Number Product was previously known as Acumedia Product: 7210 TCBS Agar; and LabM Product: LAB096 T.C.B.S. Cholera Medium



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