Sugar Free Agar

Item No.  NCM0265A

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Sugar Free Agar is used for the enumeration of psychrotrophic and mesophilic Gram-negative rods, and is not intended for use in the diagnosis of disease or other conditions in humans. A formula described by the International Dairy Federation for the enumeration of psychrotrophic and mesophilic Gram negative rods in butter and other dairy products. The Gram negative rods are able to deaminate proteins as a carbon source, whilst some enterococci are inhibited by this formula. The medium conforms to the formulation of the International Dairy Federation (I.D.F.).
Analyte Bacillus, Coliforms, E. coli, Pseudomonas spp.
Brand NEOGEN Culture Media
Formula Liter
Gelatin Peptone 7.5 g/L
Tryptone 7.5 g/L
Sodium Chloride 5.0 g/L
Agar 14.0 g/L
Grams per Liter 34.00
Liters per 500g 15.00
Mass 500 g
Package Dimensions 10.20 IN x 10.20 IN x 11.90 IN
Package Weight 15.30 LB
Plates per 500g 800.00
  1. Suspend 34 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as LabM Product: LAB087 Sugar Free Agar




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