The E. coli Vial detection system is a peptone yeast extract base with lactose as the carbon source. The selective agents include bile salts, sodium lauryl sulfate, and other Gram-positive inhibitors. Acidification of the medium due to lactose utilization changes the pH. Bromocresol purple (BCP) is used as the pH indicator. A more concentrated inhibitory system and 44.5°C temperature add to the medium's selectivity. The BCP changes from purple to yellow as acid is produced from E. coli metabolism (vials look similar to coliform vials). In an AOAC Research Institute Performance Tested MethodSM study, Soleris® for E. coli was found to be an effective method for detection of E. coli in the following sample types: cocoa powder, echinacea, mozzarella cheese, pasteurized liquid egg, condensed milk and frozen green beans. Up to 1 mL of a 1:10 sample homogenate may be added to the Soleris vial for all commodities except echinacea, where a further 1:10 dilution is required to avoid matrix inhibition of E. coli growth.
Specifications
Analyte E. coli
Approvals AOAC RI approved
Brand Soleris®
Incubation Time 14-24 Hours
Limit of Detection Dilute to Specification or Presence/Absence (with pre-incubation)
Package Dimensions 11.90 IN x 11.10 IN x 2.70 IN
Package Weight 3.70 LB
Platform Rapid Microbiology
Quantity per Package 100 vials
Result Type Quantitative
Volume 5 ml

  1. Soleris Automated System

Dependent on sample tested:

  1. Sterile 1N to 5N NaOH and/ or HCL
  2. pH meter or pH paper
  3. Butterfield's Phosphate Buffer, 99 mL (BPB-99)
  4. Tryptic Soy Broth (TS-124)

Alternate E.coli confirmation

  1. TT-123 Tryptone (Indole) Broth
  2. KO-122 Kovac's Reagent
  3. Dehydrated Tryptic Soy Agar (Acumedia® 7100)
  4. Premade Tryptic Soy Agar Plate (Remel R01917)
  5. Coli Complete Disk (Biocontrol 10800)
  6. Incubator set 35°C

Training

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