The Direct Pseudomonas Vial (PD-109) is a screening vial for the detection of Pseudomonas species. The Direct Pseudomonas Vial (PD-109) with the addition of the supplement (S2-PSI) is specific for the detection of Pseudomonas aeruginosa. The vial has an assay time of 20 hours for most applications. Following a salmonella selective enrichment protocol, organisms grow in the vial broth medium. The carbon dioxide (CO2) produced diffuses through a membrane layer into a soft agar plug containing a dye indicator. The color change in the dye is read by the instrument. The membrane layer in the vial servers as a barrier, eliminating product interference with the reading window.
Specifications
Analyte Pseudomonas
Brand Soleris®
Incubation Time 20-48 Hours
Limit of Detection Presence/Absence or Dilute to Specification
Package Dimensions 12.00 IN x 11.00 IN x 2.70 IN
Package Weight 4.40 LB
Platform Rapid Microbiology
Quantity per Package 100 vials
Result Type Quantitative
Volume 9 mL

  1. Soleris® Automated System (BSX128, BSX32 or BLX-32)
  2. PD-109, Pseudomonas Vial, 9mL (box of 100)
  3. Tryptic Soy Broth, 90 mL (BLX-TSB90)
    1. If required, use a designated neutralization broth, such as D/E Neutralizer, TAT Broth, Modified Letheen Broth, etc.

Dependent on sample tested:

  1. Sterile 1 N to 5 N sodium hydroxide (NaOH) and/or hydrochloric acid (HCl)
  2. pH meter or pH paper
  3. S2-PSI, S2 Pseudomonas Supplement

Confirmation Step:

  1. Oxidase Strips (BLX-OX)

Additional Information

20 hours for most applications for Pseudomonas aeruginosa, 48 hours for Pseudomonas species

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