Reveal® for E. coli O157:H7

Item No.  9750

  • 8-hour enrichment for 25 g samples; 12-hour enrichment for 375 g samples; 20-hour enrichment for both 25 g and 375 g samples
  • Irradiated media for easy preparation
$292.00
$279.00
$211.00
In stock
Out of stock
Expect availability in 2-4 weeks
The Reveal® for E. coli O157:H7 test system provides for the rapid recovery of Escherichia coli O157:H7 in foods, allowing detection and presumptive identification of the organism in as little as 8 hours for 25 g samples.
Specifications
Analyte E. coli O157:H7
Approvals AOAC Official Method (including 375 g for raw beef product)
  • No. 2000.13
  • No. 2000.14 (20-hour enrichment)
Brand Reveal®
Limit of Detection 1 cfu/analytical unit
Package Dimensions 13.60 IN x 7.80 IN x 4.30 IN
Package Weight 1.00 LB
Platform Lateral Flow
Quantity per Package 20 tests
Result Type Presence/Absence
Testing Time 15.00 Minutes
Time to Result 12 - 20 Hours

  1. 20 Reveal for E. coli O157:H7 test devices
  2. 20 transfer pipettes
  1. Stomacher-type bags (Neogen® item 9736)
  2. Sample bags, 3500 mL (Neogen item 9738)
  3. Scale (Neogen item 9427)
  4. Timer (Neogen item 9426)
  5. Sterile water

For 8-hour test system:

  1. 13 x 100 mm polypropylene or glass tubes (Neogen item 9438)
  2. Incubator capable of maintaining 42 ± 1°C (Neogen item 9735)
  3. Disposable sterile pipettes capable of transferring 5 mL samples (Neogen item 9415)
  4. Water bath or equivalent heating source (e.g., Bunsen burner, heater block (Neogen item 9412), microwave oven)

For 20-hour test system:

  1. Incubator capable of maintaining 36 ± 1°C (Neogen item 9735)

Optional Materials

  1. Stomacher 400 machine or equivalent

This system can utilize various enrichment media to provide E. coli O157:H7 with readily available nutrients and other factors required for its survival and rapid growth. Used with Reveal® media, the system can provide results within 8 or 20 hours for 25 g samples, or results within 12 or 20 hours for 375 g samples.

A portion (120 µL) of the enrichment culture is placed into the round sample port of the Reveal test device. This sample is wicked through a reagent zone that contains specific antibodies (anti-E. coli O157:H7) conjugated to colloidal gold particles. If target antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigen-antibody complex then leaves the reagent zone and travels through the nitrocellulose membrane that contains a zone of anti-E. coli O157:H7 antibody. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it will eventually become deposited into a waste reservoir.

The reagent zone also contains gold conjugate of a proprietary antigen (color indicator), which is eluted by the sample solution regardless of the presence of E. coli O157:H7 antigen. The gold-conjugated control indicator migrates through the membrane to the negative control capture zone (antibody to the proprietary antigen), where it is captured and aggregated to form a visible line. Regardless of the presence or absence of the E. coli O157:H7 antigen, the control line will form in the control zone, ensuring the test is working properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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