The Reveal® 2.0 for Listeria test provides for the rapid recovery of Listeria in food and environmental samples allowing next day detection and presumptive identification of Listeria.
Specifications
Analyte Listeria
Approvals AOAC-RI 041101
Brand Reveal®
Limit of Detection 1 cfu/analytical unit
Package Dimensions 0.50 IN x 6.00 IN x 1.00 IN
Package Weight 0.90 LB
Platform Lateral Flow
Quantity per Package 20 tests
Result Type Presence/Absence
Testing Time 20.00 Minutes
Time to Result 27 - 30 Hours

  1. 20 Reveal 2.0 for Listeria test devices
  2. 20 transfer pipettes
  3. 20 sample cups
  1. Incubator capable of maintaining 30 ± 1°C (Neogen® item 9735)
  2. Water bath or heater block at 80°C (Neogen items 9411, 9412
  3. 13 x 100 mm glass test tubes (Neogen item 9438)
  4. Pipettor capable of delivering 200 µL (Neogen item 9276) or transfer pipettes
  5. Sterile purified water

Test Systems:

NOTE: The Reveal 2.0 for Listeria device requires the use of certain enrichment media and supplies. These can be purchased as part of a test system or individually.

  1. Reveal 2.0 for Listeria one-step system for foods (contains devices, LESS medium, sampling bags and transfer pipettes for 20 tests) (Neogen item 9807)
  2. Reveal 2.0 for Listeria one-step complete system for environmental samples (contains devices, LESS medium, environmental sampling kits and transfer pipettes for 20 tests) (Neogen item 9806)
  3. Reveal 2.0 for Listeria 48-hour enrichment complete system (contains devices, Half Fraser Broth Plus medium, BLEB medium, sampling bags and transfer pipettes for 20 tests) (Neogen item 9808)

Media:

NOTE: Media can be purchased individually or as part of a test system.

  1. 20 foil pouches of LESS media unitized for environmental samples (Neogen item 9792E)
  2. 20 foil pouches of LESS media unitized for foods (Neogen item 9798)
  3. 20 foil pouches of Half Fraser Broth Plus (Neogen item 9782)
  4. 20 bottles of BLEB (Neogen item 9783)

Optional Materials

  1. Stomacher 400 machine or equivalent

This system utilizes Listeria enrichment single-step (LESS) medium to selectively enrich Listeria species present in food and environmental samples.

This system also uses Half Fraser Broth Plus and buffered Listeria enrichment broth (BLEB) to selectively enrich for Listeria species in select food matrices.

A sample (200 µL) equilibrated to room temperature after heat-kill of the enrichment culture is placed into the sample cup. The test device is placed into the sample and allowed to develop at ambient temperature for 20 minutes. The sample is wicked through a reagent zone that contains specific anti-Listeria antibodies conjugated to colloidal gold particles. If antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigen-antibody complex then leaves the reagent zone and travels through the nitrocellulose membrane, which contains a zone of anti-Listeria antibodies. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it is deposited into a waste reservoir.

The reagent zone also contains gold conjugate of a proprietary antigen that is eluted by the sample solution regardless of the presence of Listeria antigen. The gold-conjugated control indicator migrates through the membrane to the control capture zone (antibody to the proprietary antigen), where it is captured and aggregated to form a visible line. In the presence or absence of the Listeria antigen, the control line will form in the control zone, ensuring the test is working properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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