Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements. Rappaport-Vassiliadis Salmonella Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopoeia for the enrichment and selective isolation of Salmonella spp.from non-sterile samples. Malachite green and magnesium chloride act as selective agents, combined with high osmotic pressure and low pH to effectively inhibit non-target competitive organisms. Soya peptone provides a source of nitrogen and potassium phosphate acts as a buffer. Some Salmonella (S. typhi and S. enteritidis) are known to be sensitive to malachite green and as such may fail to grow. If these organisms are suspected an alternative selective enrichment broth (e.g. NCM0126 Mueller-Kauffmann Tetrathionate novobiocin Broth - MKTTn) should be used in parallel. This formulation is hygroscopic and will produce a slight exothermic reaction when mixed with water. According to the Harmonized Pharmacopoeia, Rappaport Vassiliadis Salmonella Enrichment Broth is used as a selective enrichment broth, with subculture performed onto Xylose Lysine Deoxycholate (XLD) agar (NCM0027).
