Rappaport-Vassiliadis Medium with Soya (RVS) (ISO)

Rappaport-Vassiliadis Medium with Soya is used as a selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. RVS is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al. originally described the broth, which was later modified by Vassiliadis et al. and optimized by van Schothorst et al. Enzymatic digest of soya provides a source of nitrogen and amino acids and sodium chloride provides osmotic pressure. The potassium phosphate components act as a buffer. Magnesium chloride and malachite green act as selective agents against non-target microorganisms. The formulation is very hygroscopic and will produce a slight exothermic reaction when mixed with water. According to ISO 6579-1:2017 subculture is performed from Buffered Peptone Water (BPW) into RVS, followed by subculture onto Xylose-Lysine Deoxycholate Agar (XLD) and another agar. This medium conforms to the performance and formulation requirements of ISO 6579-1:2017.