Item No.  NCM0049A

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PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.

Analyte Listeria spp.
Brand NEOGEN Culture Media
Formula Liter
Peptone 23.0 g/L
Yeast Extract 5.0 g/L
Lithium Chloride 10.0 g/L
Esculin 0.8 g/L
Ferric Ammonium Citrate 0.5 g/L
Mannitol 5.0 g/L
Phenol Red 0.08 g/L
Lecithin 1.0 g/L
Polysorbate 80 2.0 g/L
Grams per Liter 47.00
Liters per 500g 9.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Suspend 47 grams of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes. Cool to 45-50ºC.
  4. Aseptically add 10 mL of PALCAM Supplement (#7987).
  5. Dispense into sterile tubes.
Previous Item Number Product was previously known as Acumedia Product: 7370 PALCAM Broth; and LabM Product: LAB144 PALCAM Broth