Oxford Listeria Agar

Item No.  NCM0056A

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Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water. Oxford Listeria Agar is prepared according to the formulation of Curtis et al. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters.
Analyte Listeria monocytogenes
Brand NEOGEN Culture Media
Formula Liter
Columbia Blood Agar Base 39.0 g/L
Esculin 1.0 g/L
Ferric Ammonium Citrate 0.5 g/L
Lithium Chloride 15.0 g/L
Agar 2.0 g/L
Grams per Liter 57.50
Liters per 500g 9.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 470.00
Platform Dehydrated Culture Media

Oxford Listeria Agar

  1. Suspend 57.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C.
  5. Aseptically add 2 vials of Oxford Listeria Supplement (7986) or Oxford Supplement (X123) and mix well before dispensing.

Modified Oxford Listeria Agar

Perform steps 1-4 above and aseptically add a filtered sterilized aqueous solution of 10 mg colistin sulfate and 20 mg moxalactam. Mix well before dispensing.

Previous Item Number Product was previously known as LabM Products: LAB122 Listeria Isolation Medium (Oxford) & LAB206 Listeria Isolation Media




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