OGYE Agar Base

Item No.  NCM0132A

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OGYE Agar Base is used with oxytetracycline in the detection and isolation of yeasts and molds from foods in a laboratory setting. OGYE Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Mossel, Visser, and Mengerink described Oxytetracycline-Glucose Yeast Extract (OGYE) Agar for the detection and enumeration of yeasts and molds in foods and other specimens of sanitary significance. Mossel et al. described improved recovery of yeasts and molds compared to acidified agar. This medium is supplemented with oxytetracycline as a selective agent, rather than relying on the low pH of acidified agar to suppress bacterial growth. Acidified agar is commonly used for detection of yeasts and molds in foods and dairy products, and OGYE Agar Base provides an alternate medium.  
Specifications
Analyte Yeast & Mold
Brand NEOGEN Culture Media
Formula
Formula Liter
Yeast Extract 5.0 g/L
Dextrose 20.0 g/L
Agar 12.0 g/L
Grams per Liter 37.00
Liters per 500g 14.00
Package Dimensions 4.60 IN. x 2.90 IN x 8.60 IN.
Package Weight 1.35 IN
Plates per 500g 740.00
Preparation
  1. Suspend 37 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 115°C for 10 minutes.
  4. Cool to 45-50°C and aseptically add 2 vials of X089 Oxytetracycline Selective Supplement.
  5. Mix thoroughly and pour into Petri dishes.

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