Nutrient Broth No. 2

Item No.  NCM0189A

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Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans.

This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.

Analyte Bacillus, Campylobacter spp., E. coli, Pseudomonas spp., Salmonella, Staphylococcus
Brand NEOGEN Culture Media
Formula Liter
Beef Extract 10.0 g/L
Peptone 10.0 g/L
Sodium Chloride 5.0 g/L
Grams per Liter 25.00
Liters per 500g 20.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Suspend 19.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.
Previous Item Number Product was previously known as LabM Product: LAB014 Nutrient Broth No. 2




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