MacConkey Broth

Item No.  NCM0060A

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MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.  
Analyte E. coli
Brand NEOGEN Culture Media
Formula Liter
Pancreatic Digest of Gelatin 20.0 g/L
Lactose Monohydrate 10.0 g/L
Dehydrated Ox Bile 5.0 g/L
Bromocresol Purple 0.01 g/L
Grams per Liter 35.00
Liters per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 35 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Dispense into tubes containing Durham tubes.
  4. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7185 MacConkey Broth; and LabM Product: HP005 MacConkey Broth (USP/EP/JP)