Letheen Agar Base Modified without Tween

Item No.  NCM0130A

$105.50
$970.50
$1,970.00
$9,595.00
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Letheen Agar Base Modified (without Tween) is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base Modified is not intended for use in the diagnosis of disease or other conditions in humans.

In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.

Letheen Agar Base Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Agar Base. Letheen Agar Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.

Specifications
Analyte Non-selective
Brand NEOGEN Culture Media
Formula
Formula Liter
Letheen Agar Base 25.0 g/L
Enzymatic Digest of Casein 10.0 g/L
Enzymatic Digest of Animal Tissue 10.0 g/L
Yeast Extract 2.0 g/L
Sodium Chloride 5.0 g/L
Sodium Bisulfite 0.1 g/L
Grams per Liter 52.10
Liters per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 520.00
Preparation
  1. Suspend 52.1 g of the medium and 7 mL of Tween 80 (Polysorbate 80) in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7496 Letheen Broth Base, Modified

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