Iron Sulphite Agar

Item No.  NCM0221A

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Iron Sulphite Agar is a medium for the detection of thermophilic anaerobic organisms causing sulphide spoilage in food, and is not intended for use in the diagnosis of disease or other conditions in humans.

This formulation is a modification of Cameron Sulphite Agar, which was developed by the National Canners Association of America (now the Grocery Manufacturers Association). Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes. Beerens, and later Mossel, demonstrated that some strains of C. sporogenes would not tolerate sodium sulphite levels of 0.1%. Mossel further observed that reducing sulphite content to 0.05% improved detection of these strains.

Tryptone provides nitrogen and other nutrients necessary to support bacterial growth. The presence of sulphite reducing bacteria is indicated by the formation of black colonies. These colonies form when bacteria reduce sulphite to sulphide, which reacts with iron (III) citrate to yield a black precipitate.

Specifications
Analyte Clostridium, Desulfotomaculum, Thermophilic, Anaerobes
Brand NEOGEN Culture Media
Formula
Formula Liter
Tryptone 10.0 g/L
Sodium Sulphite 0.5 g/L
Iron (III) Citrate 0.5 g/L
Agar 12.0 g/L
Grams per Liter 23.00
Liters per 500g 22.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 1,190.00
Preparation
  1. Suspend 23 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as LabM Product: LAB222 Iron Sulphite Agar

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