HC Agar Base

Item No.  NCM0155A

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HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.

Analyte Molds
Brand NEOGEN Culture Media
Formula Liter
Enzymatic Digest of Casein 2.5 g/L
Enzymatic Digest of Animal Tissue 2.5 g/L
Yeast Extract 5.0 g/L
Dextrose 20.0 g/L
Disodium Phosphate 3.5 g/L
Monopotassium Phosphate 3.4 g/L
Ammonium Chloride 1.4 g/L
Sodium Carbonate 1.0 g/L
Magnesium Sulfate 0.06 g/L
Chloramphenicol 0.1 g/L
Agar 15.0 g/L
Grams per Liter 54.50
Liters per 500g 9.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 500.00
  1. Suspend 54.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Add 20 mL of Tween 80/Polysorbate 80 (7992) and mix.
  4. Autoclave at 121°C for 15 minutes.
  5. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7520 HC Agar Base