GeneQuence® for Listeria

Item No.  6708

  • 24 and 48-hour enrichment protocols for food and environmental samples
  • Tests can be performed manually or automated
$1,238.00
In stock
Out of stock
Expect availability 12/11/2020
GeneQuence® for Listeria provides for the detection of Listeria in food and environmental samples.
Specifications
Analyte Listeria
Approvals
  • AOAC-RI 010403
  • HPB Canada MLFP-13
Brand GeneQuence®
Incubation Time 30-48 Hours
Limit of Detection 1 - 5 cfu/25g sample cfu
Package Dimensions 9.70 IN x 6.50 IN x 4.50 IN
Package Weight 0.70 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including sulfuric acid (mists), which is known in the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 93 tests
Result Type Qualitative
Testing Time 2.00 Hours

  1. 1 bottle of pretreatment concentrate (labeled 1a)
  2. 1 bottle of 12 mL pretreatment buffer (labeled 1b)
  3. 1 bottle of lysis reagent concentrate (labeled 2a)
  4. 1 bottle of 12 mL lysis reagent buffer (labeled 2b)
  5. 1 bottle of 18 mL hybridization solution (labeled 3)
  6. 1 bottle of 5 mL Listeria probe solution (labeled 4)
  7. 1 bottle of 50 mL wash solution 20X concentrate (labeled 5)
  8. 1 bottle of 15 mL substrate chromogen solution (labeled 6)
  9. 1 bottle of 5 mL stop solution (labeled 7)
  10. 1 bottle of 5 mL positive control (labeled +)
  11. 1 bottle of 5 mL negative control (labeled -)
  12. 1 48-well microwell plate in divisible strips
  13. Hybridization/probe mixture dilution chart

Available from Neogen

Equipment

  1. Angled thermometer for digital heat block (item #9382)
  2. Apparatus for plate washing with vacuum source (Manual item #9348, Automated item #6718 or #6718A)
  3. Automated plate wash assembly: vacuum tubing assembly (item #9387), side arm flask assembly (item #6822), vacuum pump (item #6824)
  4. Carboy w/spigot (2) (item #9349)
  5. Digital heat block (item #9386)
  6. Heated block cover (item #9383)
  7. Incubators at 30°C ± 1° and 35°C ± 1° (item #9735)
  8. Microwell reader to read at 450 nm with discrimination of 0.01 absorbance unit (item #6704, #6704A or #9302)
  9. Modular heating block titer plate (item #9384)
  10. Water bath at 37°C (item # 9346)

Optional Equipment

  1. Dry heater base incubator (item #9412)
  2. 8-channel pipettor & tips to dispense 50-300 µL (item #9385, #9407)
  3. Heater block at 37°C ± 1° (item #9411)

Lab Supplies & Consumables

  1. Micropipette & tips to dispense 20-200 µL volumes (item #9276, #9407)
  2. Minute timer (item #9426)
  3. Test tubes, 12 x 75 mm borosilicate glass, and rack (items #9439 and #9440)
  4. Stomacher bags(100) (item #9736)
  5. 50 mL culture tubes for sample pre-enrichment (item #9381)
  6. 1000 mL graduated cylinder (item #9345)
  7. 500 mL wash bottle (item #9366)
  8. 10 mL pipette pump (item #9277)
  9. 10 mL sterile serological pipettes, 0.1 mL graduations (item #9415)
  10. Eppendorf reservoir (item #4018)
  11. Centrifuge tube racks (item #9479)

Media

  1. BLEB Base Supplements (item #7980)
  2. Buffered Listeria Enrichment Broth Base (BLEB Base, item #7675)
  3. LESS Enrichment Broth (item #9790A)
  4. Oxford Listeria Agar Base (item #7428)
  5. Oxford Medium Antimicrobics, FDA (ref. 1, item #7986)
  6. PALCAM Broth Base (ref. 3, item #7670)
  7. PALCAM Supplement (ref. 3, item #7987)
  8. UVM Modified Listeria Enrichment Broth, UVM (ref. 2, item #7409)

Not available from Neogen

  1. Homogenizer (e.g., Stomacher)
  2. Modified Oxford Medium (MOX), FSIS (ref. 2)
  3. Petri plates, 100 x 15 mm
  4. Phosphate-Buffered Saline (PBS), 10 mM sodium phosphate, 0.85% sodium chloride, pH 7.4
  5. Sodium Pyruvate Supplement (10% aqueous solution, ref. 1)
  6. Sterile cotton swabs and absorbent paper
  7. 2 mL disposable graduated pipettes

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more