GeneQuence® for Listeria monocytogenes

1. 1 bottle of pretreatment concentrate (labeled 1a)
2. 1 bottle of 12 mL pretreatment buffer (labeled 1b)
3. 1 bottle of lysis reagent concentrate (labeled 2a)
4. 1 bottle of 12 mL lysis reagent buffer (labeled 2b)
5. 1 bottle of 18 mL hybridization solution (labeled 3)
6. 1 bottle of 5 mL L. mono probe solution (labeled 4)
7. 1 bottle of 50 mL wash solution 20X concentrate (labeled 5)
8. 1 bottle of 15 mL substrate chromogen solution (labeled 6)
9. 1 bottle of 5 mL stop solution (labeled 7)
10. 1 bottle of 5 mL positive control (labeled +)
11. 1 bottle of 5 mL negative control (labeled -)
12. 1 96-well microwell plate in divisible strips
13. Hybridization/probe mixture dilution chart

Available from Neogen

Equipment

1. Angled thermometer for digital heat block (item #9382)
2. Apparatus for plate washing with vacuum source (Manual item #9348, Automated item #6718 or #6718A)
3. Automated plate wash assembly: vacuum tubing assembly (item #9387), side arm flask assembly (item #6822), vacuum pump (item #6824)
4. Carboy w/spigot (2) (item #9349)
5. Digital heat block (item #9386)
6. Heated block cover (item #9383)
7. Incubators at 30°C ± 1° and 35°C ± 1° (item #9735)
8. Microwell reader to read at 450 nm with discrimination of 0.01 absorbance unit (item #6704, #6704A or #9302)
9. Modular heating block titer plate (item #9384)
10. Water bath at 37°C (item # 9346)

Optional Equipment

1. Dry heater base incubator (item #9412)
2. 8-channel pipettor & tips to dispense 50-300 µL (item #9385, #9407)
3. Heater block at 37°C ± 1° (item #9411)

Lab Supplies & Consumables

1. Micropipette & tips to dispense 20-200 µL volumes (item #9276, #9407)
2. Minute timer (item #9426)
3. Test tubes, 12 x 75 mm borosilicate glass, and rack (items #9439 and #9440)
4. Stomacher bags(100) (item #9736)
5. 50 mL culture tubes for sample pre-enrichment (item #9381)
6. 1000 mL graduated cylinder (item #9345)
7. 500 mL wash bottle (item #9366)
8. 10 mL pipette pump (item #9277)
9. 10 mL sterile serological pipettes, 0.1 mL graduations (item #9415)
10. Eppendor reservoir (item #4018)
11. Centrifuge tube racks (item #9479)

Media

1. BLEB Base Supplements (item #7980)
2. Buffered Listeria Enrichment Broth Base (BLEB Base, item #7675)
3. LESS Enrichment Broth (item #9790A)
4. Oxford Listeria Agar Base (item #7428)
5. Oxford Medium Antimicrobics, FDA (ref. 1, item #7986)
6. PALCAM Broth Base (ref. 3, item #7670)
7. PALCAM Supplement (ref. 3, item #7987)
8. UVM Modified Listeria Enrichment Broth, UVM (ref. 2, item #7409)

Not available from Neogen

1. Homogenizer (e.g., Stomacher)
2. Modified Oxford Medium (MOX), FSIS (ref. 2)
3. Petri plates, 100 x 15 mm
4. Phosphate-Buffered Saline (PBS), 10 mM sodium phosphate, 0.85% sodium chloride, pH 7.4
5. Sodium Pyruvate Supplement (10% aqueous solution, ref. 1)
6. Sterile cotton swabs and absorbent paper
7. 2 mL disposable graduated pipettes