Fraser Broth

Item No.  NCM0050A

$85.50
$787.00
$1,599.00
$3,735.00
$7,780.00
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Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.

Specifications
Analyte Listeria spp.
Brand NEOGEN Culture Media
Formula
Formula Liter
Enzymatic Digest of Animal Tissues 5.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Meat Extract 5.0 g/L
Yeast Extract 5.0 g/L
Esculin 1.0 g/L
Disodium Hydrogen Phosphate 9.6 g/L*
Potassium Dihydrogen Phosphate 1.35 g/L
Sodium Chloride 20.0 g/L
Lithium Chloride 3.0 g/L
Acriflavine 0.025 g/L
Nalidixic Acide 0.02 g/L
*Equivalent to Disodium Hydrogen Phosphate Dihydrate 12.0 g/L
Grams per Liter 55.00
Liters per 500g 9.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 55 grams of the medium in 1 liter of purified water.
  2. Mix thoroughly
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.
  5. Aseptically add 2 vials of Ferric Ammonium Citrate Supplement (X211) or 2 vials of Fraser Broth Base Supplement (7984) and mix thoroughly before dispensing.
Previous Item Number Product was previously known as Acumedia Product: 7502 Fraser Broth Base; and LabM Product: LAB212 Fraser Broth PLUS

Additional Information

Ferric Ammonium Citrate Supplement (X211) or Fraser broth base supplement (7984) may be used

Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin also. Enzymatic digest of animal tissues, casein, meat extract and yeast extract provide the essential vitamins, minerals, amino acids, nitrogen and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization. According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO) and then sub-cultured onto Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.