Fastidious Anaerobe Agar

Item No.  NCM0014A

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Fastidious Anaerobe Agar is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. A primary isolation medium capable of growing most clinically significant anaerobes. Developed by Lab M (Neogen® Corporation), comparisons have shown this medium to be superior to other formulations as a primary isolation medium for fastidious organisms. The peptones included have been chosen for maximum growth stimulation. Starch and sodium bicarbonate act as de-toxification agents while hemin encourages pigment production in Porphyromonas melaninogenicus. Specific growth promoting agents are Cysteine for Fusobacterium necrophorum, Propionibacterium acne and Bacteriodes fragilis, arginine for Eubacterium spp. soluble pyrophosphate for Porph. gingivalis and Porph. asaccharolyticus. Pyruvate helps neutralize hydrogen peroxide and is also utilized by Veillionella spp. as an energy source. Vitamin K and sodium succinate provide essential growth factors for some anaerobes as does the 0.1% glucose. The low level of glucose prevents the production of high levels of acids and alcohols which would inhibit colonial development.  
Specifications
Analyte Actinomyces, Anaerobes, Bacteroides, Brucella, Clostridium, Eubacterium, Fusobacterium, Haemophilus, Helicobacter, Neisseria, Peptostreptococcus, Porphyromonas, Propionibacterium, Streptococci
Brand NEOGEN Culture Media
Formula
Formula Liter
Peptone Mix 23.0 g/L
Sodium Chloride 5.0 g/L
Soluble Starch 1.0 g/L
Agar 12.0 g/L
Sodium Bicarbonate 0.4 g/L
Glucose 1.0 g/L
Sodium Pyruvate 1.0 g/L
Cysteine HCl Monohydrate 0.5 g/L
Hemin 0.01 g/L
Vitamin K 0.001 g/L
L-Arginine 1.0 g/L
Soluble Pyrophosphate 0.25 g/L
Sodium Succinate 0.5 g/L
Grams per Liter 46.00
Liters per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 590.00
Preparation
  1. Suspend 45.7 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Prepare 5 to 10% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45 – 50°C.
Previous Item Number Product was previously known as Acumedia Product: 7531 Fastidious Anaerobe Agar; and LabM Product: LAB090 Fastidious Anaerobe Agar