Eosin Methylene Blue (EMB) Agar Levine

Item No.  NCM0105A

$86.50
$794.00
$1,613.00
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Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.

This medium was introduced in 1916 by Holt-Harris and Teague to differentiate Escherichia spp. and Aerobacter spp. It was modified by Levine in 1918 who removed sucrose from the formula and increased the lactose content. The distinctive metallic sheen produced by E. coli on this medium is due to acid production resulting in an amide bonding between the eosin and methylene blue, other coliforms do not produce enough acid to cause this reaction. Eosin inhibits most Gram-positive organisms. The prepared medium is sensitive to light.

Specifications
Analyte Candida, E. coli, Staphylococci
Brand NEOGEN Culture Media
Formula
Formula Liter
Peptone 10.0 g/L
Lactose 10.0 g/L
Dipotassium Hydrogen Phosphate 2.0 g/L
Eosin Yellow 0.4 g/L
Methylene Blue 0.065 g/L
Agar 15.0 g/L
Grams per Liter 37.50
Liters per 500g 14.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 790.00
Preparation
  1. Suspend 37.5 grams of the medium and add to 1 liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and agitate gently to ensure uniform distribution of the flocculant precipitate which is a feature of this medium before pouring into Petri dishes.
  5. STORE IN THE DARK.
Previous Item Number Product was previously known as Acumedia Product: 7103 Eosin Methylene Blue Agar, Levine; and LabM Product: LAB061 Eosin Methylene Blue Agar (Levine)

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