DC Medium with BCIG
Item No.  NCM0112A
$429.50
$3,947.00
$7,978.00
$39,028.00

DC Medium with BCIG

Item No.  NCM0112A

Learn more
$429.50
$3,947.00
$7,978.00
$39,028.00
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 0
To order or for more information contact us

DC Medium with BCIG is used in the chromogenic differentiation of E. coli from other coliforms in water samples using the membrane filtration method in a laboratory setting. DC Medium with BCIG is not intended for use in the diagnosis of disease or other conditions in humans.

DC (Differential Coliform) Medium with BCIG is a selective and differential medium for the presumptive identification of E. coli. This medium is enhanced by the addition of a chromogenic agent, BCIG, 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide to detect glucuronidase activity. The presence of the enzyme ß-D-glucuronidase differentiates most E. coli spp. from other coliforms, and is the same enzyme used in the MUG reaction. BCIG reacts slightly differently, and when released into the medium is insoluble, accumulating within the cell, imparting a blue to purple color to presumptive E. coli colonies.

Specifications
Analyte Coliforms, E. coli
Brand NEOGEN Culture Media
Formula
Formula Liter
Lactose 10.0 g/L
Tryptose 10.0 g/L
Yeast Extract 3.0 g/L
Sodium Chloride 5.0 g/L
Proteose Peptone 5.0 g/L
Bile Salts 1.5 g/L
BCIG 0.2 g/L
Neutral Red 0.08 g/L
Agar 15.0 g/L
Grams per Liter 49.80
Liters per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 550.00
Preparation
  1. Suspend 49.8 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45 - 50°C.
  5. Aseptically add 1.2 mL of a sterile 10 mg/mL solution of Cefsulodin and mix well.
Previous Item Number Product was previously known as Acumedia Product: 7704 DC Medium with BCIG

Support

Get in Touch

You may also like

NEOGEN Ampouled Media, MI Broth
Item No. 6510

Developed and approved by the U.S. EPA for the detection of Total Coliforms and E. coli in drinking water for MI Broth only.

$97.00

View
Lauryl Tryptose (LST) Broth

$72.00

View
MacConkey Broth
MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements.

$92.50

View
NEOGEN Ampouled Media, m-ENDO Broth
Item No. 6500
Used for enumerating coliforms and recommended by the American Public Health Association for testing water, wastewater, and foods following the U.S. EPA water test method.

$66.00

View
Reveal® for Multi-Treenut
Item No. 8555
Reveal® for Multi-Treenut Test is intended for the qualitative analysis of treenut (almond, cashew, hazelnut, pecan, pistachio, and walnut) residue in clean-in-place rinses and on environmental surfaces.

$250.00

View
NEOGEN Ampouled Media, m-Green Yeast and Fungi Broth
Item No. 6505
Used for the detection of yeast and fungi in beverages.

$66.00

View
NEOGEN Ampouled Media, m-TGE Broth
Item No. 6515
Used for the determination of bacterial counts and specified by the American Public Health Association for the heterotrophic plate count procedure in testing bottled water.

$66.00

View
Egg Yolk Tellurite - 7983
Item No. 7983
Egg Yolk Tellurite, 100 mL is a sterile suspension of stabilized egg yolk in saline with potassium tellurite used as an enrichment in bacterial culture media in a laboratory setting.

$49.00

View