Columbia CNA Agar

Item No.  NCM0115A

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Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.

Analyte Gram-positive cocci
Brand NEOGEN Culture Media
Formula Liter
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 8.0 g/L
Enriched Peptone 10.0 g/L
Starch 1.0 g/L
Sodium Chloride 5.0 g/L
Colistin 0.015 g/L
Nalidixic Acid 0.01 g/L
Agar 14.0 g/L
Grams per Liter 43.00
Liters per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 630.00
  1. Suspend 43 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Do not overheat medium.
  4. Cool to 45 - 50°C.
  5. Prepare 5% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium.
Previous Item Number Product was previously known as Acumedia Product: 7126 Columbia CNA Agar