Columbia Blood Agar Base

Item No.  NCM0031A

$95.50
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Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms.  
Specifications
Analyte Non-selective
Brand NEOGEN Culture Media
Formula
Formula Liter
Columbia peptone mixture 25.1 g/L
Soluble starch 1.0 g/L
Sodium chloride 5.0 g/L
Agar 12.0 g/L
Grams per Liter 43.10
Liters per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 630.00
Preparation
  1. Suspend 43.1 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45–50°C and add 5-7% sterile defibrinated horse or sheep blood.
  5. Mix well before dispensing into sterile Petri dishes.
Previous Item Number Product was previously known as Acumedia Product: 7125 Columbia Blood Agar Base; and LabM Product: LAB215 Columbia II Agar Base

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