Columbia Agar

Item No.  NCM0013A

$125.00
$1,147.00
$2,318.00
$11,339.00
In stock
Out of stock
Expect availability 09/30/2020
Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Clostridia from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Originally described as a general purpose nutritious agar base by Ellner et al. at Columbia University that can be enriched by the addition of sterile blood. The peptone mixture and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. The Harmonized European Pharmacopoeia states that where necessary, gentamicin sulfate at a concentration of 20mg/L can be added after sterilization to reduce the growth of non-target organisms. According to the Harmonized European Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.  
Specifications
Analyte Clostridium spp.
Brand NEOGEN Culture Media
Formula
Formula Liter
Pancreatic Digest of Casein 10.0 g/L
Meat Peptic Digest 5.0 g/L
Heart Pancreatic Digest 3.0 g/L
Yeast Extract 5.0 g/L
Maize Starch 1.0 g/L
Sodium Chloride 5.0 g/L
Agar 12.0 g/L
Grams per Liter 41.00
Liters per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 620.00
Preparation
  1. Dissolve 41 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7734 Columbia Agar; and LabM Product: HP012 Columbia Agar (USP/EP/JP)