Clostridium Difficile Agar Base

Item No.  NCM0128A

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Clostridium Difficile Agar Base (Brazier’s) is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile. Clostridium Difficile is not intended for use in the diagnosis of disease or other conditions in humans.

Brazier’s CCEY agar is the formulation currently used by the Anaerobe Reference Unit for the isolation of C. difficile, resulting from work initiated by Ken Phillips and Paul Levett, and completed by Jon Brazier. Based upon the market leading anaerobe medium, Fastidious Anaerobe Agar, it incorporates additional ingredients to improve the isolation and differentiation of C. difficile from samples.

Cholic acid is present to promote spore germination following alcohol shock treatment, and p-hydroxyphenylacetic acid to enhance the production of p-cresol, a distinctive metabolite of C. difficile. Selectivity is achieved by addition of supplement X093 (cefoxitin cycloserine) and egg yolk emulsion X075 is added to help differentiate C. difficile from lecithinase positive clostridia. Finally, the addition of lysed horse blood optimizes the recognition of colony fluorescence when cultures are examined using UV light.

Specifications
Analyte Clostridium difficile
Brand NEOGEN Culture Media
Formula
Formula Liter
Peptone Mix 23.0 g/L
Sodium Chloride 5.0 g/L
Soluble Starch 1.0 g/L
Agar 12.0 g/L
Sodium Bicarbonate 0.4 g/L
Glucose 1.0 g/L
Sodium Pyruvate 1.0 g/L
Cysteine HCl 0.5 g/L
Hemin 0.01 g/L
Vitamin K 0.001 g/L
L-Arginine 1.0 g/L
Soluble Pyrophosphate 0.25 g/L
Sodium Succinate 0.5 g/L
Cholic Acid 1.0 g/L
p-Hydroxyphenylacetic acid 1.0 g/L
Grams per Liter 48.00
Liters per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 570.00
Preparation
  1. Suspend 48 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C and aseptically add the following: 2 vials of Cycloserine/Cefoxitin Selective Supplement X093, 40 mL of Egg Yolk Emulsion X073 and 10 mL lysed horse blood.
  5. Mix well and dispense.
Previous Item Number Product was previously known as Acumedia Product: 7385 Clostridium difficile Agar; and LabM Product: LAB160 Brazier's CCEY Agar

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