Brilliant Green Agar with Sulfapyridine

Item No.  NCM0133A

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Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.

Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.

Analyte Salmonella spp.
Brand NEOGEN Culture Media
Formula Liter
Yeast Extract 3.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 5.0 g/L
Sodium Chloride 5.0 g/L
Lactose 10.0 g/L
Sucrose 10.0 g/L
Brilliant Greent 0.0125 g/L
Phenol Red 0.08 g/L
Sodium Sulfapyridine 1.0 g/L
Agar 20.0 g/L
Grams per Liter 59.00
Liters per 500g 8.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 460.00
  1. Suspend 59 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Avoid overheating.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7299 Brilliant Green Agar w/ Sulfapyridine