Baird Parker Agar Base

Item No.  NCM0200A

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Baird Parker Agar is used for the cultivation of Staphylococcus aureus from foodstuffs according to ISO 6888, and is not intended for use in the diagnosis of disease or other conditions in humans. Originally introduced in 1962, this medium was developed by Baird-Parker to overcome the problems of recovering damaged Staphylococcus aureus from foodstuffs. This version of the medium is formulated according to ISO 6888-1:1999+A1:2003 and is in compliance with ISO 6888-2:2003+A1:2003 and ISO 6888-3:2003. Baird-Parker medium is highly selective by nature, due to the presence of potassium tellurite and lithium chloride. Tellurite inhibits most coliforms and is also reduced to telluride by S. aureus, giving the typical black colonies. Glycine and sodium pyruvate are both used as growth factors by staphylococci while the pyruvate also neutralizes any toxic peroxides that may be formed. When Baird-Parker medium is used with Egg Yolk Tellurite X085, presumptive S. aureus appear as black colonies demonstrating lecithinase activity (an opaque zone around the colony) and lipase activity (a zone of clearing encircling the opaque zone). Suspected S. aureus colonies should be confirmed with RPF for coagulase or latex agglutination test. Rabbit plasma fibrinogen (RPF X086) is a more specific alternative to egg yolk tellurite and allows the direct detection of coagulase-positive S. aureus. Typical S. aureus appear as black colonies surrounded by a zone of precipitation (demonstrating coagulase activity). This is recognized as the gold standard method for the identification of S.aureus. RPF overcomes any issues with atypical colony forms and its use means further confirmatory tests are not necessary.
Specifications
Analyte Staphylococcus spp.
Brand NEOGEN Culture Media
Formula
FormulaLiter
Pancreatic Digest of Casein10.0 g/L
Yeast Extract1.0 g/L
Meat Extract5.0 g/L
Sodium Pyruvate10.0 g/L
L-Glycine12.0 g/L
Lithium Chloride5.0 g/L
Agar20.5 g/L
Grams per Liter 63.50
Liters per 500g 8.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 430.00
Preparation

For Baird-Parker Medium with Egg Yolk Tellurite X085

  1. Suspend 63.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121oC for 15 minutes.
  4. Cool to 45-50°C and add 5% (50mL) X085.
  5. Mix well before aseptically pouring into sterile Petri dishes.
  6. Dry the agar surface prior to use. Sulphamezathine may be added at 0.05g/L to suppress the swarming of Proteus spp.

For Baird-Parker Medium with Rabbit Plasma Fibrinogen (RPF) Supplement X086

  1. Suspend 6.35 grams of the medium in 90 ml of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121oC for 15 minutes.
  4. Cool to 45-50°C and add 1 vial of reconstituted X086.
  5. Mix well before aseptically pouring into sterile Petri dishes.
  6. Dry the agar surface prior to use.
Previous Item Number Product was previously known as LabM Product: LAB285 Baird Parker Medium Base (ISO)

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