Bacillus cereus MYP Agar

Item No.  NCM0062A

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Bacillus Cereus Mannitol Egg Yolk (MYP) Agar is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. NCM0062 conforms to ISO and FDA / BAM formulation. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Formulated according to ISO 7932:2004, MYP was introduced by Mossel and his co-workers in 1967 for the enumeration of Bacillus cereus in foods and was shown to be the most effective for this purpose by Inal in 1972. B. cereus is differentiated from other members of the Bacillus group by two reactions: mannitol fermentation and lecithinase production. Mannitol fermentation on this medium produces a yellow color, due to the indicator dye, phenol red changing color as acid is produced. B. cereus is mannitol negative and produces red colonies. The lecithinase production of B. cereus is indicated by a white precipitate around the colonies. Polymyxin is added to suppress coliforms but some Proteus spp. and Gram-positive cocci may grow.

Specifications
Analyte Bacillus cereus
Brand NEOGEN Culture Media
Formula
Formula Liter
Beef Extract 1.0 g/L
Peptone 10.0 g/L
D-Mannitol 10.0 g/L
Sodium Chloride 10.0 g/L
Phenol Red 0.025 g/L
Agar 15.0 g/L
Grams per Liter 46.00
Liters per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 590.00
Preparation
  1. Suspend 46 grams of the medium in 950 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C and supplement per method below.
  5. Aseptically add 50 mL of NCM4017* Egg Yolk Emulsion and 2 vials of NCM4032-0.5* Polymyxin B Supplement each reconstituted using 5 mL of sterile deionized/RO water.

Preparation per FDA BAM

  1. Suspend 46 grams of the medium in 900 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 - 50°C and supplement per method below.
  5. Aseptically add 50 mL of NCM4017* Egg Yolk Emulsion and 2 vials of NCM4032-0.5* Polymyxin B Supplement each reconstituted using 5 mL of sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7741 Bacillus Cereus MYP Agar

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