ANSR® for Listeria

Item No.  9871

  • 16 – 26 hour enrichment protocols for food (25 g and 125 g) and environmental samples
  • Irradiated media for easy preparation
$1,109.00
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Expect availability in 2-4 weeks
ANSR® for Listeria provides rapid recovery and detection of Listeria in environmental and food samples in 16 – 26 hours.
Specifications
Analyte Listeria
Approvals AOAC PTM 101202
Brand ANSR®
Incubation Time 16-26 Hours
Limit of Detection 1 cfu/analytical unit
Package Dimensions 7.70 IN x 5.20 IN x 3.90 IN
Package Weight 0.80 LB
Platform Molecular
Quantity per Package 96 tests
Result Type Qualitative
Testing Time 18.00 Minutes

  1. 12 strips of 8 cluster tubes, 1.2 mL
  2. 12 strips of 8 reaction tubes, 200 µL, containing lyophilized ANSR for Listeria reagents in 2 sealed foil pouches with desiccant pack
  3. 12 strips of 8 permanent caps for reaction tubes
  4. 1 bottle lysis reagent suspension buffer, 60 mL
  5. 3 vials containing lyophilized lysis reagents
  6. 1 kit insert

Equipment Required

  1. ANSR reader (Neogen item 9828)
  2. Computer and software for connection to ANSR reader (Neogen item 9832)
  3. 1 dual heater block with lysis block inserts for 1.2 mL cluster tubes, 80 ± 2°C and 37 ± 2°C (Neogen items 9386-DUAL48, 9829-48)
  4. Pipettor, 100 – 1000 µL (Neogen item 9463)
  5. Pipette tip rack, 100 – 1000 µL, sterile, 96 tips (Neogen item 9487)
  6. Pipettor, 10 – 100 µL, 8-channel (Neogen item 9388)
  7. Pipette tips, 100 µL, sterile, filtered, 96 tips (Neogen item 9389)
  8. Vortex mixer, adjustable speed (Neogen item 9494)
  9. Stomacher (optional)
  10. 3 thermometers, traceable (Neogen item 9518)
  11. Timer, 3-channel (Neogen item 9426)
  12. Optional heater block with block insert for 0.2 mL reaction tubes, 56 ± 1°C (Neogen items 9386-48D, 9829-64)
  13. Webcam (Neogen item WEBCAM)
  14. ANSR ethernet cable (Neogen item 9835)
  15. Pipette pump, 10 mL (Neogen item 9277)
  16. Pippettes, sterile serological, 10 mL (Neogen item 8686)
  17. 40-slot, 20 mm test tube rack, autoclavable (Neogen item 9553)
  18. Pipettor, 20-200 µL (Neogen item 9276)

Other Materials Required

  1. Stomacher-type bags for sample enrichment. Filtered bags are recommended (Neogen item 6827)
  2. Graduated cylinder, 250 mL (Neogen item 9368)

Media Enrichment Broth Required

  1. ANSR for Listeria Enrichment Broth 1 (Neogen item 9814) - for environmental samples
  2. LESS Medium, 500 g bottle (Neogen item 9790A) - for food samples except meats
  3. Reveal® Buffered Listeria Enrichment Broth (BLEB), 500 g bottle (Neogen item 9786) - for meat samples. Also used for confirmation, see page 6 of Kit Insert.
  4. LESS Plus Medium, 500 g bottle (Neogen item 9880) - for select matrices.
  5. 1 L purified water
  6. Phosphate Buffered Saline (if testing smoked salmon or guacamole) (Neogen item 8425)

  1. A special lysis reagent is added to the enrichment sample. Target pathogen DNA or RNA is released through the lysis step.
  2. The lysed sample is added to ANSR reagents. A special primer targets specific regions of the pathogen DNA and starts amplification of the DNA, creating millions of copies in minutes.
  3. The amplified segments of the pathogen DNA bind to special molecular beacons which then fluoresce and are detected by the ANSR reader.
  4. Results will be reported in 18 minutes for and displayed as positive, negative or invalid.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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