ANSR® for E. coli O157:H7

ANSR for E. coli O157:H7

Item No.  9822

  • Total time to results 12 – 26 hours
  • Irradiated media for easy preparation
  • Validated for raw beef trim (375 g), raw ground beef (325 g), spinach and sprout irrigation water
$1,176.00
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Expect availability in 2-4 weeks
ANSR® for E. coli O157:H7 provides rapid recovery and detection of E. coli O157:H7 in food samples in 12 – 26 hours.
Specifications
Analyte E. coli O157:H7
Approvals AOAC 111502
Brand ANSR®
Incubation Time 12-26 Hours
Limit of Detection 1 cfu/analytical unit
Package Dimensions 8.00 IN x 5.40 IN x 4.40 IN
Package Weight 1.20 LB
Platform Molecular
Quantity per Package 96 tests
Result Type Qualitative
Testing Time 10.00 Minutes

  1. 12 strips of 8 cluster tubes, 1.2 mL
  2. 12 strips of 8 reaction tubes, 200 µL, containing lyophilized ANSR for E. coli O157:H7 reagents in 2 sealed foil pouches with desiccant pack
  3. 12 strips of 8 permanent caps for reaction tubes
  4. 1 bottle lysis reagent suspension buffer, 60 mL
  5. 3 vials containing lyophilized lysis reagents
  6. 1 kit insert

Equipment Required

  1. ANSR reader (Neogen item 9828)
  2. Computer and software for connection to ANSR reader (Neogen item 9832)
  3. 1 dual heater block with lysis block inserts for 1.2 mL cluster tubes, 80 ± 2°C and 37 ± 2°C (Neogen items 9386-DUAL48, 9829-48)
  4. Pipettor, 100 – 1000 µL (Neogen item 9463)
  5. Pipette tip rack, 100 – 1000 µL, sterile, 96 tips (Neogen item 9487)
  6. Pipettor, 10 – 100 µL, 8-channel (Neogen item 9388)
  7. Pipette tips, 100 µL, sterile, filtered, 96 tips (Neogen item 9389)
  8. Vortex, adjustable speed (Neogen item 9494)
  9. Stomacher (optional)
  10. 3 thermometers, traceable (Neogen item 9518)
  11. Timer, 3-channel (Neogen item 9426)
  12. Optional heater block with block insert for 0.2 mL reaction tubes, 56 ± 1°C (Neogen items 9386-48D, 9829-64)
  13. Webcam (Neogen item WEBCAM)
  14. ANSR ethernet cable (Neogen item 9835)
  15. Pipette pump, 10 mL (Neogen item 9277)
  16. Pippettes, sterile serological, 10 mL (Neogen item 8686)
  17. 40-slot, 20 mm test tube rack, autoclavable (Neogen item 9553)
  18. Pipettor, 20-200 µL (Neogen item 9276)

Other Materials Required

  1. Stomacher-type bags for sample enrichment. Filtered bags are recommended (Neogen item 6833)
  2. Graduated cylinder, 250 mL (Neogen item 9368)

Media Enrichment Broth Required

  1. ANSR for E. coli Enrichment Broth (Neogen item 9815)
  2. Tryptic Soy Broth, Modified with Acid Digest of Casein (mTSB) (Neogen item 7167A)
  3. 1 L purified water
  4. Phosphate Buffered Saline (Neogen item 8425)

  1. A special lysis reagent is added to the enrichment sample. Target pathogen DNA or RNA is released through the lysis step.
  2. The lysed sample is added to ANSR reagents. A special primer targets specific regions of the pathogen DNA and starts amplification of the DNA, creating millions of copies in minutes.
  3. The amplified segments of the pathogen DNA bind to special molecular beacons which then fluoresce and are detected by the ANSR reader.
  4. Results will be reported in 10 minutes and displayed as positive, negative or invalid.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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