Wilkins-Chalgren Agar - 7232

Item No.  7232A

This item has been replaced by item NCM0106.
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Wilkins-Chalgren Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Wilkins-Chalgren Agar is not intended for use in the diagnosis of disease or other conditions in humans. This medium was selected because it does not require the addition of blood to support satisfactory growth of most anaerobes. The survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature.  
Brand Acumedia®
Formula Liter
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Gelatin 10 g
Yeast Extract 5 g
Sodium Chloride 5 g
Dextrose 1 g
L-Arginine 1 g
Sodium Pyruvate 1 g
Hemin 0.005 g
Vitamin K 0.0005 g
Agar 15 g
Mass 500 g
Package Dimensions 3.60 IN x 3.60 IN x 6.50 IN
Package Weight 0.50 LB
  1. Suspend 48 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.




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