National Food Laboratory Aseptic Validation Medium - 7737

Item No.  7737A

This item has been replaced by item NCM0007.
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National Food Laboratory Aseptic Validation Medium is used for the cultivation of mesophilic or thermophilic spoilage microorganisms in food in a laboratory setting. National Food Laboratory Aseptic Validation Medium is not intended for use in the diagnosis of disease or other conditions in humans. Foods may spoil either due to biological or chemical reasons. Spore-forming bacteria, e.g., Clostridium and Bacillus, are important because of their heat resistant (thermophilic) nature. In addition, there are other microorganisms which are not heat resistant (mesophilic), but enter through the leakage of the container during cooling. Examples of spoilage type thermophilic microorganisms are flat-sour and sulfide spoilage and can be found in corn, peas and spinach. Mesophilic microorganisms include putrefactive anaerobes, butyric anaerobes, and aciduric flat-sour, and can be found in tomatoes and peas. In the 1930's, the National Canners Association specified use of Dextrose Tryptone Agar for isolating "flat sour" organisms from food products. National Food Laboratory Aseptic Validation Medium is a general purpose medium for the recovery of aerobic and facultative organisms used in the validation and commissioning of aseptic filling systems. This medium is ideally suited for the spore-forming organisms used in the validation of steam, hydrogen peroxide, and peracetic acid sterilization systems.  
Brand Acumedia®
Formula Liter
Enzymatic Digest of Casein 10 g
Dextrose 5 g
Yeast Extract 1 g
Bromcresol Purple 0.04 g
Mass 500 g
Package Dimensions 3.60 IN x 3.60 IN x 6.50 IN
Package Weight 0.50 LB

Laboratory Use

  1. Dissolve 16 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

In-Plant Commissioning Use

  1. In mix system dissolve 16 grams of the medium per liter of water.
  2. Agitate for 20 minutes to dissolve the medium.
  3. Process at minimum F0 = 8.0 (hold-tube process).




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