Letheen Broth Base, Modified - 7496

500 g

Item No.  7496A

This item has been replaced by item NCM0129.
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Letheen Broth Base, Modified is used with Polysorbate 80 for the recovery of microorganisms from cosmetics in a laboratory setting. Letheen Broth Base, Modified is not intended for use in the diagnosis of disease or other conditions in humans. In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test. Letheen Broth Base, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. Letheen Broth Base, Modified is recommended by the FDA for use in the microbiological testing of cosmetics.  
Brand Acumedia®
Formula Liter
Letheen Broth Base 20.7 g
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 10 g
Yeast Extract 2 g
Sodium Bisulfite 0.1 g
Mass 500 g
Package Dimensions 3.60 IN x 3.60 IN x 6.50 IN
Package Weight 0.50 LB
  1. Dissolve 37.8 g of the medium and 5 g of Tween 80 (Polysorbate 80) (#7992) in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.




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