Brain-Heart Infusion Broth - 7116

10 kg

Item No.  7116C

This item has been replaced by item NCM0016.
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Brain-Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms in a laboratory setting. Brain-Heart Infusion Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rosenow prepared a rich medium for culturing streptococci by combining dextrose broth and brain tissue. Hayden modified the original formula while working with dental pathogens. The current formula is a modification of Rosenow and Hayden, using dehydrated infusions of porcine brain and heart tissue. Brain-Heart Infusion Broth can be supplemented with antibiotics, varying amounts of sodium chloride, yeast extract, and serum to provide a rich medium for bacteria, yeasts and pathogenic fungi. The addition of 0.1% agar can be used to lower oxygen tension, providing an atmosphere to support the growth of aerobic, microaerophilic, and obligate anaerobic microorganisms. Brain-Heart Infusion Broth, abbreviated as BHI, is specified in many references for food and water testing.  
Brand NEOGEN Culture Media
Formula Liter
Brain Heart Infusion 17.5 g
Enzymatic Digest of Gelatin 10 g
Dextrose 2 g
Sodium Chloride 5 g
Disodium Phosphate 2.5 g
Mass 10 kg
Package Dimensions 2.50 IN x 11.10 IN x 15.70 IN
Package Weight 26.30 LB
  1. Dissolve 37 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.




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