Using 16S for microbiome applications allows for incredible resolution in food spoilage or plate microbiome applications. 16S metagenomic analysis allows for some critical improvements over traditional subtyping methods.

Microbiomes

Ecological community of commensal, symbiotic, and pathogenic microorganisms in a similar space.
Key issues with traditional methods

  • VBNC – viable but not culturable
  • Media biases
  • Anaerobic organisms

Unbiased Information

16S metagenomics doesn’t use culture methods to islate bacteria for identification. Circumventing this step with metagenomics provides a more complete and accurate picture of the microbial community.

Powerful Data

Metagenomics provides insights and statistics for processors unobtainable through traditional methods. 16S Metagenomics can be used for:

  • Spoilage investigations
  • Shelf life determination
  • Sanitizing verification and validation

Send us the samples

We use Ilumnia sequencers at our GeneSeek Laboratory to generate data to answer big questions like:

  • Spoilage point source
  • Shelf life limiting ingredients
  • Sanitization chemical rotations
Specifications
Brand NeoSeek™

Additional Information

16S vs. Traditional Methods

  • Traditional methods suffer from two specific issues: volume of work, and media biases.
  • The volume of work through subtyping a sample can be enormous. Each different organism needs to be isolated, and then use a traditional biochemical identification. In an environmental sample from food production, this could mean 15-20 isolates!
  • The other element is viable but not culturable. Traditional subtyping methods introduce a bias by only selecting organisms that can grow in the media used in isolation. 16S circumvents this issue by obtaining amplifying and identifying the 16S sequence in a neat sample (no isolation).

Metagenomics for Food Safety and Quality Webinars

Metagenomics has many different applications in food industry. We developed a webinar series to illustrate some of the applications and differences between other next generation sequencing technologies (i.e. whole genome sequencing).

  • 16S Metagenomics: A Case Study for Trouble-shooting Spoilage Sources
  • Demystifying the S: WGS vs 16S
  • Arming Your Scientists for Clean Label Formulations
Watch On-Demand

The key elements for food safety genomics include three critical areas. These three aspects are leveraged with NeoSeek™ through various partners and existing laboratories.

Sequencing technology

Illumina is the platform used for most of the NGS applications used in the NeoSeek Assays. These sequencers have allowed the data acquisition costs to drop dramatically. This now allows the service to be implemented on a larger scale and beyond the research laboratories used at universities and other research organizations.

GeneSeek® lab and capabilities

GeneSeek in Lincoln, NE is the largest animal genomics laboratory in the world. NeoSeek uses the existing infrastructure to process samples quickly and with high quality results. This ISO 17025 lab has been delivering genetic results for over a decade.

Bioinformatics

Leveraging custom built pipelines and curated databases, the bioinformatics is a critical element to generating meaningful, and high quality results. The bioinformatics also helps structure data to generate meaningful and actionable results.

NEOGEN® is the exclusive licensee of pipelines and analytics from Metagenome Analytics (MGA). These services provide the bioinformatics development and deployment for the NeoSeek Assays. MGA consists of the world’s leading experts in food microbiome and food safety applications of next generation sequencing.

Bioinformatics is the process of assembling and interpreting sequence data for applications such as 16S metagenome sequencing, whole genome sequencing, or whole genome metagenomics. The millions of reads that accompany each application needs computing power and programs to assemble the sequences, and interpret the data. MGA maintains curated databases, proprietary bioinformatics pipelines, and customer reporting features to help deliver the key insights possible with next generation sequencing.