Veratox® for Gentamicin is a competitive enzyme immunoassay for the quantitative analysis of gentamicin in muscle. Veratox for Gentamicin enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect gentamicin in muscle and to satisfy customer concerns about food safety.
Specifications
Analyte Gentamicin
Brand Veratox®
Package Dimensions 5.60 IN x 3.50 IN x 5.20 IN
Package Weight 0.70 LB
Platform ELISA
Quantity per Package 36 tests
Range of Detection 0.50 ppb - 27.00 ppb
Result Type Quantitative
Testing Time 60.00 - 120.00 Minutes
Time to Result 45 - 60 Minutes

  1. Gentamicin-coated Plate 1 x 96-well plate (8 wells x 12 strips)
  2. Gentamicin Standards:
    1. 0 ng/mL (white cap tube) 0.8 mL
    2. 0.050 ng/mL (yellow cap tube) 0.8 mL
    3. 0.10 ng/mL (orange cap tube) 0.8 mL
    4. 0.30 ng/mL (pink cap tube) 0.8 mL
    5. 0.90 ng/mL (purple cap tube) 0.8 mL
    6. 2.7 ng/mL (blue cap tube) 0.8 mL
    7. Gentamicin Spiking Stock 50.0 ng/mL (red cap tube) 0.8 mL
  3. Gentamicin Antibody #1 12 mL
  4. 100X HRP-Conjugated Antibody #2 250 µL
  5. Antibody #2 Diluent 20 mL
  6. 10X Sample Extraction Buffer 25 mL
  7. 20X Wash Solution 28 mL
  8. Stop Buffer 14 mL
  9. TMB Substrate 12 mL
  10. 10X Gentamicin Extraction Buffer A 12 mL
  11. 1X Gentamicin Balance Buffer 1.2 mL
  1. Microtiter plate reader with a 450 nm filter (Neogen® item 9303)
  2. 20–200 µL pipettes (Neogen item 9276)
  3. Multichannel pipette: 50–300 µL (optional) (Neogen item 9385)
  4. 1000 µL pipettor (Neogen 9337)
  5. Vortex Mixer (Neogen 9494)
  6. Centrifuge 4,000 x g
  7. Timer (Neogen item 9426)
  8. Wash bottle (Neogen item 9400)
  9. Paper towels or equivalent absorbent material
  10. Veratox software (Neogen item 9305)
  11. Lab station (Neogen item 9481)
  12. Distilled or deionized water
  13. Blender or food processor to homogenize sample

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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Validation

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