Veratox® for Chloramphenicol
Item No. 9551
- Rapid results easily determined by reader
- Low levels of detection for Minimum Required Performance Limit (MRPL)
| Specifications | |
|---|---|
| Analyte | Chloramphenicol |
| Brand | Veratox® |
| Limit of Detection | 0.02 ng/mL or 10 ppt |
| Package Dimensions | 6.10 IN x 3.10 IN x 4.10 IN |
| Package Weight | 1.50 LB |
| Quantity per Package | 90 tests |
Documents
Technology
Veratox for Chloramphenicol is an enzyme-linked immunosorbent assay (ELISA). The basis of this test is a competition between a chloramphenicol-enzyme conjugate and any free chloramphenicol from the sample for a limited number of binding sites on the antibody-coated microplate wells.
Samples are extracted, and the extracts (and solutions of chloramphenicol standards) are added to the microplate wells. A solution of the chloramphenicol-enzyme conjugate is also added to each of the wells. At this point, time is allowed for any free chloramphenicol and the chloramphenicol-enzyme conjugate to compete for the binding sites on the plate. Next, the plate is washed to remove unbound material and a substrate solution is added to each well. During an ensuing reaction period, any bound enzyme conjugate reacts with the substrate to produce a blue-colored solution. The color-forming reaction is terminated by adding Stop Solution to the wells of the plate, turning the blue color to yellow. The absorbance of each well is then measured using a microplate reader set at 450 nm. The extent of color formation is inversely proportional to the amount of chloramphenicol present in that well.
Samples that contain little or no chloramphenicol will have a relatively large absorbance while a sample that contains more chloramphenicol will have a smaller absorbance. Quantitative results may be obtained by comparing the absorbance of the samples to a standard curve generated from the wells containing the chloramphenicol standards.
Training
Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.