Veratox® for Chloramphenicol
Item No.  9551
$610.50

Veratox® for Chloramphenicol

Item No.  9551

  • Rapid results easily determined by reader
  • Low levels of detection for Minimum Required Performance Limit (MRPL)
Learn more
$610.50
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 0
To order or for more information contact us
Materials Required
Pipette Tips, 1-200 µL
Item No. 9410

$46.00

Materials Required
Pipettor, 100 µL Fixed
Item No. 9272

$254.00

Materials Required
Timer

$12.00

View
Veratox® for Chloramphenicol is used for the quantitative analysis of chloramphenicol residues in shrimp. The test is a competitive direct ELISA that provides exact concentrations in parts per trillion (ppt). Free chloramphenicol in the sample and controls competes with enzyme-labeled chloramphenicol (conjugate) for the antibody binding sites. After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color. A microwell reader is used to yield optical densities. Control optical densities form a standard curve, and sample optical densities are plotted against the curve to calculate the exact concentration of chloramphenicol.
Specifications
Analyte Chloramphenicol
Brand Veratox®
Limit of Detection 0.02 ng/mL or 10 ppt
Package Dimensions 6.10 IN x 3.10 IN x 4.10 IN
Package Weight 1.50 LB
Quantity per Package 90 tests
  1. 1 plate (96 wells) of antibody-coated microwells
  2. 1 vial of chloramphenicol 0.0 ng/mL standard, 2 mL
  3. 1 vial of chloramphenicol 0.02 ng/mL standard, 2 mL
  4. 1 vial of chloramphenicol 0.05 ng/mL standard, 2 mL
  5. 1 vial of chloramphenicol 0.1 ng/mL standard, 2 mL
  6. 1 vial of chloramphenicol 0.5 ng/mL standard, 2 mL
  7. 1 vial of chloramphenicol 2 ng/mL standard, 2 mL
  8. 1 bottle of ready-to-use chloramphenicol enzyme conjugate, 20 mL
  9. 1 bottle of sample dilution buffer, 100 mL
  10. 1 bottle of wash buffer concentrate, 100 mL (dilute 1:10 with deionized water before use)
  11. 1 bottle of substrate solution, 28 mL
  12. 1 bottle of stop solution, 28 mL
  1. Pipettor, 100 µL (Neogen® item #9272)
  2. Pipettor, multichannel, 100 µL
  3. Pipette tips for 100 µL and multichannel pipettors (Neogen item #9410)
  4. 3 reagent boats for multichannel pipettor
  5. Paper towels or equivalent absorbent material
  6. Pipettor capable of measuring 1.0 mL
  7. Pipettor capable of measuring 0.5 mL (for isooctane/chloroform)
  8. Graduated or volumetric pipettor capable of measuring 6.0 mL of solvent
  9. Disposable pipettes capable of measuring 4.0 mL of solvent
  10. Glass test tubes capable of being centrifuged at 2,000 g (16 x 100 mm preferred)
  11. Caps for glass test tubes
  12. Ethyl Acetate (ACS reagent grade or better)
  13. Chloroform
  14. Isooctane (2,2,4-trimethylpentane)
  15. Deionized or distilled water
  16. Blender or food processor
  17. Centrifuge capable of 2,000 g, swinging bucket rotor preferred
  18. Heating block
  19. Compressed air or nitrogen and necessary tubing
  20. Water bath
  21. Vortex
  22. Automated plate washer
  23. Microplate reader with 450 nm filter (650 nm reference filter is optional)
  24. Balance capable of measuring 3.0 g
  25. Timer (Neogen item #9426)
  26. Graduated cylinder to dilute wash buffer
  27. Waterproof marker
  28. Pipette aid for dispensing solvents
  29. Stainless steel spatulas for transferring shrimp into test tubes
  30. Thermometer

Technology

Veratox for Chloramphenicol is an enzyme-linked immunosorbent assay (ELISA). The basis of this test is a competition between a chloramphenicol-enzyme conjugate and any free chloramphenicol from the sample for a limited number of binding sites on the antibody-coated microplate wells.

Samples are extracted, and the extracts (and solutions of chloramphenicol standards) are added to the microplate wells. A solution of the chloramphenicol-enzyme conjugate is also added to each of the wells. At this point, time is allowed for any free chloramphenicol and the chloramphenicol-enzyme conjugate to compete for the binding sites on the plate. Next, the plate is washed to remove unbound material and a substrate solution is added to each well. During an ensuing reaction period, any bound enzyme conjugate reacts with the substrate to produce a blue-colored solution. The color-forming reaction is terminated by adding Stop Solution to the wells of the plate, turning the blue color to yellow. The absorbance of each well is then measured using a microplate reader set at 450 nm. The extent of color formation is inversely proportional to the amount of chloramphenicol present in that well.

Samples that contain little or no chloramphenicol will have a relatively large absorbance while a sample that contains more chloramphenicol will have a smaller absorbance. Quantitative results may be obtained by comparing the absorbance of the samples to a standard curve generated from the wells containing the chloramphenicol standards.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Support

Get in Touch

LabLive Remote Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more

You may also like

Agri-Screen Ticket Activator Ampoules
For use with Agri-Screen Tickets

$12.00

View
Ractopamine LFD Kit for Swine Urine
Item No. RT-LFD-25-SU-IDS
The Ractopamine LFD is a qualitative one-step competitive inhibition immunoassay for the detection of Ractopamine in animal urine.  It detects the presence of Ractopamine at 2.5 ppb or higher concentrations.

$195.00

View
Complete Ractopamine STARTER LFD Kit
Item No. RT-STK-LFD-F-IDS

$78.50

View
Agri-Screen® Tickets
Item No. 8950
Agri-Screen® Ticket is used to detect all major organophosphates, thiophosphates, and carbamates in air, water, soil, produce, food, spills, surfaces and custom applications.

$85.50

View
Tryptic Soy Agar (Soybean-Casein Digest Agar)
Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP.

$73.50

View
DSP Hydrolysis Pack
Item No. 9554

$38.00

View
Filter Syringes
Item No. 9420

$107.00

View
Pipettor, 100-1000 µL
Item No. 9463

$471.00

View