Ammonium Chloride

Item No.  NCM0178A

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Ammonium Chloride is used in the preparation of Minerals Modified Glutamate Agar (NCM0179) and Minerals Modified Glutamate Broth (NCM0186). Ammonium chloride is not intended for use in the diagnosis of disease or other conditions in humans. Ammonium Chloride can be used in the preparation of two key media. Minerals Modified Glutamate Agar is a highly defined medium recommended in ISO 16649-1:2018, containing various amino acids, vitamins and salts formulated to promote the recovery of stressed or damaged organisms. According to ISO 16649-1 organisms are inoculated onto a cellulose membrane overlaid on MMGA for a resuscitation stage, followed by transfer of the membrane onto Tryptone Bile Glucuronide Agar (TBX) for isolation and counting. Minerals Modified Glutamate Broth was developed for use with the Most Probable Numbers Technique (M.P.N.) for the enumeration of coliforms in water supplies. The medium is an improved version of the chemically defined glutamic acid medium described by Gray in 1964. The product is supplied in three parts because it has been shown that separating the sodium glutamate from the base improves its stability.
Specifications
Analyte Coliforms, E. coli
Brand NEOGEN Culture Media
Formula
Formula Liter
Ammonium Chloride 100%
Mass 500 g
Package Weight 1.35 LB
Preparation

Preparation in Minerals Modified Glutamate Broth:

  1. Suspend 23.5 grams of base medium together with 6.35 grams of sodium glutamate (NCM0181) in one liter of purified water containing 2.5 grams of ammonium chloride (NCM0178).
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C.

Preparation in Minerals Modified Glutamate Broth

Double Strength:

  1. Dissolve 22.7 grams of base medium (NCM0186) together with 12.7 grams of sodium glutamate (NCM0181) in one liter of purified water containing 5 grams of ammonium chloride (NCM0178).
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Dispense 10ml and 50ml volumes into tubes with inverted Durham tube.
  4. Sterilize by autoclaving for 10 minutes at 115°C. Alternatively heat to 100°C for 30 minutes on three successive days.

Single Strength:

  1. Dissolve in 11.35 grams of base medium (NCM0186) together with 6.35 grams of sodium glutamate (NCM0181) in 1 liter of distilled water containing 2.5 grams ammonium chloride (NCM0178).
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Dispense 5ml volumes into tubes with inverted Durham tubes.
  4. Sterilize by autoclaving for 10 minutes at 115°C, alternatively heat to 100°C for 30 minutes on three successive days.
Previous Item Number Product was previously known as Acumedia Product: LabM Product: LAB080C

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