Veratox® for Peanut Allergen is used for the quantitative analysis of peanut residues in food products such as cookies, crackers, chocolate bars, ice cream and cereals. Can also be used to screen environmental swabs.
Specifications
Analyte Peanut
Approvals AOAC-RI 030403
Brand Veratox®
Limit of Detection 1 ppm peanut
Package Dimensions 7.50 IN x 4.70 IN x 9.70 IN
Package Weight 1.40 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 2.50 ppm peanut - 25.00 ppm peanut
Result Type Quantitative
Testing Time 30.00 Minutes

  1. 48 antibody-coated microwells (24 per pouch)
  2. 48 red-marked transfer wells (24 per pouch)
  3. 5 yellow-labeled bottles of 0, 2.5, 5, 10, 25 ppm peanut controls
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution
  7. 5 foil pouches of 10 mM PBS dry powder extraction solvent. Each pouch is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  8. 40 mL of 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  9. 200 g extraction additive
  10. Plastic scoop to measure extraction additive
  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable plastic extraction bottles
    2. 20 disposable transfer pipettes
  2. Shaker water bath capable of maintaining 60 ± 1°C with clamps to hold 250 mL extraction bottles
  3. Whatman no. 4 filters or equivalent (Neogen item 9429)
  4. Centrifuge (optional)
  5. Pipettor, 50–200 µL, adjustable (Neogen item 9276)
  6. Pipettor, 12-channel (Neogen item 9273)
  7. Pipette tips (Neogen item 9410, 9407, 9417)
  8. Timer (Neogen item 9426)
  9. Microwell reader Stat-Fax with a 650 nm filter (Neogen item 9303)
  10. Bottle, 1 L, to prepare washing solution (Neogen item 9472)
  11. Bottle, 1 L heat safe, to prepare extract solution (Neogen item 9472)
  12. Paper towels or equivalent absorbent material
  13. Microwell holder (Neogen item 9402)
  14. Waterproof marker
  15. Wash bottle (Neogen item 9400)
  16. Distilled or deionized water
  17. 3 reagent boats for 12-channel pipettor (Neogen item 9435)
  18. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  19. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)

The Veratox for Peanut Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Peanut protein residue is extracted from samples with a phosphate buffered salt solution (PBS) by shaking in a heated water bath, followed by centrifugation or filtration. Extracted peanut residue is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound peanut residue is washed away and a second antibody (detector antibody), which is enzyme labeled, is added. The detector antibody binds to the already bound peanut residue. After a second wash, substrate is added. Color develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the color of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of peanut protein.

Training

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