Veratox® for Crustacea Allergen is used for the quantitative analysis of crustacea proteins in food products, ingredients and clean-in-place rinses. Can also be used to screen environmental swabs.
Specifications
Analyte Crustacea
Brand Veratox®
Limit of Detection <1 ppm crustacea ppm
Package Dimensions 10.00 IN x 8.00 IN x 3.25 IN
Package Weight 0.60 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 38 tests
Range of Quantitation 2.50 ppm crustacea - 25.00 ppm crustacea
Result Type Quantitative
Testing Time 30.00 Minutes

  1. 48 antibody-coated microwells (24 per pouch)
  2. 48 red-marked transfer wells (24 per pouch)
  3. 5 yellow-labeled bottles of 0, 2.5, 5, 10, 25 ppm crustacea controls
  4. 2 blue-labeled bottles of enzyme-labeled antibody conjugate
  5. 1 green-labeled bottle of K-Blue® Substrate
  6. 1 red-labeled bottle of Red Stop solution 7. 5 pouches of 10 mM PBS dry powder extraction buffer. Each pouch is enough to prepare 1 L in distilled or deionized water
  7. 40 mL of 25 x 10 mM PBS-Tween washing reagent in a wide mouth bottle. Each bottle is enough to prepare 1 L in distilled or deionized water (pH 7.4)
  8. 50 g of extraction additive in a specimen cup
  9. Plastic scoop to measure extraction additive
  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable plastic extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile sawbs
    2. 100 dropper tips
  3. Shaker water bath capable of maintaining 30°C with clamps to hold 250 mL disposable plastic bottles
  4. Whatman no. 4 filters or equivalent (Neogen item 9429)
  5. Centrifuge (optional)
  6. Pipettor, 50–200 µL, adjustable (Neogen item 9276)
  7. Pipettor, 12-channel (Neogen item 9273)
  8. Pipette tips (Neogen item 9410/9407)
  9. Timer (Neogen item 9426)
  10. Microwell reader with a 650 nm filter (Neogen item 9303)
  11. Bottle, 1 L, to prepare washing solution (Neogen item 9472)
  12. Bottle, 1 L, to prepare extract solution (Neogen item 9472)
  13. Paper towels or equivalent absorbent material
  14. Microwell holder (Neogen item 9402)
  15. Waterproof marker
  16. Wash bottle (Neogen item 9400)
  17. Distilled or deionized water
  18. Three reagent boats for 12-channel pipettor (Neogen item 9435)
  19. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  20. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)

Each test kit contains antibody-coated wells with antibodies specific to the kit’s target substance.

First, samples and controls are added to their respective wells. During an incubation, the target substance binds to the antibodies. The wells are washed to remove all unbound materials.

An enzyme conjugate (antibody chemically linked with an enzyme) is added to all wells. During an incubation, conjugate binds to the already bound target substance, forming a “sandwich.” The more target substance in the wells, the more conjugate that binds in the wells.

The wells are washed again to remove all unbound conjugate.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, color develops in proportion to the amount of conjugate in the wells. The more conjugate, the more color, and the more target substance that is detected.

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Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


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