Reveal® for Peanut Allergen is intended for the qualitative analysis of peanut residue in food products (e.g., cookies, crackers, chocolate bars, ice cream and cereals), clean-in-place rinses, and on environmental surfaces.
Specifications
Analyte Peanut
Brand Reveal®
Limit of Detection Screens samples at 5 ppm peanut
Package Dimensions 8.40 IN x 7.90 IN x 3.40 IN
Package Weight 0.90 LB
Platform Lateral Flow
Quantity per Package 25 tests
Result Type Qualitative
Testing Time 10.00 Minutes

  1. 25 lateral flow test strips in a sealed stay-dry tube
  2. 25 transfer pipettes
  3. 25 sample tubes
  4. 4 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  5. 2 bottles of 50 g extraction additive
  6. Plastic 3 g scoop to measure extraction additive
  1. High-speed blender with a 250 mL jar (Neogen® item #9493/#9477)
  2. Allergen Extraction Kit (Neogen item #8429)
  3. Allergen Environmental Swabbing Kit (Neogen item #8432S)
  4. Scale capable of weighing 5 ± 0.1 grams (Neogen item #9427)
  5. Water bath, hot plate or equivalent heat source capable of maintaining 60°C ± 1°
  6. Thermometer
  7. 1 L bottle to prepare extraction solution (Neogen item #9472)
  8. Waterproof marker
  9. Distilled or deionized water
  10. Timer (Neogen item #9426)
  11. Graduated cylinder capable of measuring 125 mL (Neogen item #9368)
  12. Whatman #4 filter paper (Neogen item #9429)
  13. Reveal AccuScan® III System (Neogen item #9590) optional

Assay Principle

Reveal for Peanut Allergen is a single-step lateral flow immunochromatographic assay. The extract is wicked through a reagent zone, which contains antibodies specific for peanut protein conjugated to colored particles. If peanut is present, it will be captured by the conjugated antibodies. The peanut-antibody-particle complex is then wicked onto a membrane which contains a zone of antibody specific for peanut protein. This zone captures the complex allowing the particles to concentrate and form a visible line. If no peanut is present, no line will form. The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of peanut, ensuring the strip is working properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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