Alert® for Total Milk Allergen is used as a screening tool to test raw ingredients, in clean-in-place rinses, on environmental surfaces, or product throughout the production process, for the presence of milk residue.
Specifications
Analyte Milk
Brand Alert®
Limit of Detection 5 ppm, 10 ppm NFDM
Package Dimensions 10.50 IN x 8.00 IN x 3.00 IN
Package Weight 0.60 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 20 tests
Result Type Qualitative
Testing Time 30.00 Minutes

  1. 24 antibody-coated microwells
  2. 2 yellow-labeled bottles of controls made from nonfat dried milk; 1 bottle at a concentration of 5 ppm and 1 bottle at 10 ppm (see procedural note No. 1)
  3. 1 blue-labeled bottle of enzyme-labeled antibody conjugate
  4. 1 green-labeled dropper bottle of K-Blue® Substrate solution
  5. 1 red-labeled dropper bottle of Red Stop solution
  6. 20 unlabeled empty sample dropper bottles with tips
  7. 3 foil pouches of 10 mM PBS dry powder extraction solvent; each pouch contains enough powder to prepare 1 L of extraction solution
  8. 1 wide-mouth bottle of 40 mL PBS-Tween wash buffer concentrate; a bottle contains enough concentrate to prepare 1 L of wash buffer
  9. 50 g of extraction additive in a specimen cup
  10. Plastic scoop to measure extraction additive
  1. Allergen Extraction Kit (Neogen® item 8429)
    1. 20 disposable extraction bottles
    2. 20 disposable transfer pipettes
  2. Allergen Environmental Swabbing Kit (Neogen item 8432S)
    1. 100 sterile swabs
    2. 100 disposable dropper tips
  3. Scale capable of weighing 5 ± 0.1 g (Neogen item 9427)
  4. Shaker water bath, hot plate or equivalent heat source capable of maintaining 60 ± 1°C
  5. Thermometer
  6. Two 1 L bottles to prepare washing and extraction solutions (Neogen item 9472)
  7. Paper towels or equivalent absorbent material
  8. Microwell holder (Neogen item 9402)
  9. Waterproof marker
  10. Wash bottle (Neogen item 9400)
  11. Distilled or deionized water
  12. Timer (Neogen item 9426)
  13. Graduated cylinder capable of measuring 125 mL (Neogen item 9368)
  14. Pipettor, 100 µL (Neogen item 9272, 9278, 9276)
  15. Pipette tips (Neogen item 9410, 9407, 9417)
  16. Vortex (Neogen item 9494)
  17. 50 mL sample tubes (Neogen item 9381)

First, samples and controls are added to their respective wells. During an incubation, the target substance binds to the antibodies. The wells are washed to remove all unbound materials.

An enzyme conjugate (antibody chemically linked with an enzyme) is added to all wells. During an incubation, conjugate binds to the already bound target substance, forming a “sandwich.” The more target substance in the wells, the more conjugate that binds in the wells.

The wells are washed again to remove all unbound conjugate.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, color develops in proportion to the amount of conjugate in the wells. The more conjugate, the more color, and the more target substance that is detected.

Results are read visually in a screening format—the more blue color, the more target substance detected.

Alert_diagram.png

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more