Alert® for Gliadin R5 is used as a screening tool to test for prolamins (wheat gliadin, rye secalin, and barely hordein) in a wide variety of food products, ingredients and clean-in-place rinses, and on environmental swabs.
Specifications
Analyte Gliadin/Gluten
Brand Alert®
Limit of Detection 10 ppm gliadin, 20 ppm gluten
Package Dimensions 10.50 IN x 8.00 IN x 3.00 IN
Package Weight 0.90 LB
Platform ELISA
Prop 65 Warning
Warning
WARNING: This product can expose you to chemicals including thimerosal, which is known in the State of California to cause birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov.
Quantity per Package 20 tests
Result Type Qualitative
Testing Time 30.00 Minutes

  1. 24 antibody-coated microwells
  2. 1 yellow-labeled dropper bottle of 10 ppm gliadin control (20 ng/mL gliadin)
  3. 1 blue-labeled dropper bottle of conjugate
  4. 1 green-labeled dropper bottle of K-Blue® Substrate
  5. 1 red-labeled dropper bottle of Red Stop solution
  6. 20 prefilled dilution dropper bottles of 4.9 mL PBS
  7. 1 foil pouch containing enough 10 mM PBS dry powder to prepare 1 L of dilution solvent
  8. 1 widemouth bottle containing enough 10 mM PBS-Tween wash buffer concentrate to prepare 1 L in distilled or deionized water (pH 7.4)
  9. 30 g of extraction additive in a specimen cup
  10. Plastic 1 g scoop to measure extraction additive
  1. Orbital rotator or shaker to hold 50 cc centrifuge tubes for a 1 g sample or shaker or shaker water bath with clamps adjusted to hold 125 mL (4 oz) extraction bottles for a 2 g sample
  2. Allergen Environmental Swabbing Kit (Neogen® item 8432S)
  3. Scale capable of weighing 1 + 0.1 g (Neogen item 9427), or scale capable of weighing 0.25 ± 0.01 g if using renaturing cocktail solution (Neogen item 9395)
  4. Gliadin renaturing cocktail solution for analysis of heat processed samples (Neogen item 8515, 8515S, or 8515B)
  5. 2 1 L bottles to prepare washing solution, extract solution (Neogen item 9472)
  6. Timer (Neogen item 9426)
  7. Microwell holder (Neogen item 9402)
  8. Pipettor, 100 µL (Neogen item 9272, 9278 or 9276)
  9. Pipette tips (Neogen item 9410)
  10. Wash bottle (Neogen item 9400)
  11. Paper towels or equivalent absorbent material
  12. Waterproof marker
  13. Distilled or deionized water
  14. Laboratory grade ethanol (190 proof)
  15. Centrifuge (optional)

First, samples and controls are added to their respective wells. During an incubation, the target substance binds to the antibodies. The wells are washed to remove all unbound materials.

An enzyme conjugate (antibody chemically linked with an enzyme) is added to all wells. During an incubation, conjugate binds to the already bound target substance, forming a “sandwich.” The more target substance in the wells, the more conjugate that binds in the wells.

The wells are washed again to remove all unbound conjugate.

A substrate, which changes color in the presence of the conjugate, is added to the wells. During an incubation, color develops in proportion to the amount of conjugate in the wells. The more conjugate, the more color, and the more target substance that is detected.

Results are read visually in a screening format—the more blue color, the more target substance detected.

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Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

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