BioKits for Speciation Identification

Item No.  902011Q

$679.00
$433.00
$433.00
$433.00
$433.00
$679.00
$433.00
In stock
Out of stock
Expect availability in 2-4 weeks
Biokits for Species Identification are sandwich-based ELISA assays which provide sensitive and specific qualitative detection of a range of animal species (cow, horse, pig, poultry, and sheep) in meats, meat products, and feedstuff.
Specifications
Analyte Beef, Horse, Pork, Poultry, Sheep
Brand BioKits
Limit of Detection 1 percent
Package Dimensions 7.60 IN x 5.00 IN x 3.50 IN
Package Weight 1.00 LB
Platform ELISA
Quantity per Package 96 wells
Result Type Quantitative
Testing Time 30.00 - 150.00 Minutes

RAW

Component Detail Vials / Bottle / Pack Ready to Use
RMS Control Positive for cow, pig, sheep and poultry 1
Cow Conjugate 1
Pig Conjugate 1
Sheep Conjugate 1
Poultry Conjugate 1
TMB Substrate 1
Wash solution concentrate 1 10-fold concentrate
Diluent Concentrate Type 9 1 5-fold concentrate
Stop Solution CARE: Strong Acid 1
Cow / pig / sheep / poultry plate 96 microwells
(3 x 8 well strips per species)
Red – Cow
Yellow – Pig
Green – Sheep
Blue – Poultry
N/A
Package insert N/A N/A
Blank result form N/A N/A

COOKED

Component Detail Vials / Bottle / Pack Ready to Use
Positive Controls Each serves as a positive control in the appropriate species test and can also be used as a negative control in any other (cooked) species test 4
Species Biotin Single Species Kit: two vials per species

4

Four Species Kit: one vial per species 2
Avidin Peroxidase Conjugate 1
TMB Substrate 1
Wash solution concentrate 1 10-fold concentrate
Stop Solution CARE: Strong Acid 1
Beef / Pork / Poultry / Sheep Plate Single Species Kit: 6 x 8 well strips
Four Species Kit: 96 microwells
(3 x 8 well strips per species)
Red - Beef
Yellow - Pork
Blue - Poultry
Green - Sheep
N/A
Package insert N/A N/A
Blank result form N/A N/A

RAW

Reagents

  • Sodium chloride
  • Purified water

Required Equipment

  • Miscellaneous laboratory plastic and/or glassware, plate seals, equipment for sample handling/extraction and containers suitable for meat extracts (e.g., Stomacher bags, 10 mL graduated tubes).
  • Precision micropipette(s) capable of delivering 50 and 100 µL, plus disposable tips.

Optional equipment (recommended):

  • Domestic blender or mincer or Stomacher and suitable sample preparation bags
  • Orbital microwell plate shaker set at 700–800 rpm.
  • Centrifuge and appropriate centrifuge/microfuge tubes for clarifying sample extract.
  • Microwell washer (e.g., NUNC Immuno Wash 8) or wash bottle
  • Microwell plate reader, fitted with 450 nm interference filter (calibrate regularly) or spectrophotometer and disposable semi-micro cuvettes.
  • Disposable plastic Pasteur pipettes (nominal drop size (water) 35–40 µL) for sample/reagent addition.

COOKED

Important Note: All samples to be tested must be COOKED. If uncooked samples are to be tested, or it is suspected that the sample is not fully cooked, it is necessary to heat treat the sample/saline mixture in a water bath at >95° for 15 min or a microwave for 90 seconds prior to mixing and filtration.

Reagents:

  • Sodium Chloride
  • Purified water

Equipment:

  • Domestic blender or mincer.
  • Miscellaneous laboratory plastic and/or glassware, including measuring cylinders, pipettes and containers suitable for meat extracts.
  • Whatman no. 4 (or similar) filter papers.
  • Boiling water bath (if samples require heat treatment - see above).

Microwave Extraction Method:

  • Microwave (850 W or equivalent*)
  • Stomacher

Enzyme Immunoassay:

  • Precision micropipette and disposable tips capable of delivering 50 & 100 microlitres.
  • Reagent wash bottle (for plate washing).
  • Enzyme Immunoassay (Optional):
    • Precision repeating dispenser capable of delivering 50 & 100 microlitres.
    • Microwell washer, (eg. NUNC Immuno Wash 8).
    • Spectrophotometer (set at 450 nm) and semi micro (1 ml) cuvettes.
    • Microwell plate reader, fitted with 450 nm interference filter.

*For microwaves of different power output cooking times can be adjusted as follows:
(Cooking time in seconds X Wattage of Microwave used for validation)/Wattage of Microwave to be used
e.g. (90 X 850)/750 = 102 seconds


Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more