The Science Inside: Mycotoxin Competitive ELISA

July 12, 2019

A look inside the science of Neogen's Competitive ELISA test.

Video Transcript


Mycotoxin Competitive ELISA

First, the sample and enzyme conjugate are simultaneously pipetted into the antibody-coated wells.

During the incubation, the mycotoxin and enzyme conjugate compete to bind to the capture antibody.

The well is then washed out leaving behind whatever mycotoxin or enzyme conjugate has bound itself to the capture antibody.

The substrate is added and reacts with the enzyme conjugate changing the color of the liquid.

A darker color means that fewer mycotoxin molecules remain in the well resulting in a greater amount of conjugate interacting with the substrate.

A less significant color change means that a greater amount of mycotoxins remain in the well.

The final step is adding red stop which ends the reaction and changes the color gradient.

The well's optical densities are read on a microwell reader.


Category: Science Insights