The Science Inside: Competitive Lateral Flow

July 12, 2019

A look into the science inside the competitive lateral flow chemistry.

Video Transcript

[Music]

Positive Sample

The sample is added to the lateral flow device.

Liquid traveling up the device picks up the colored-label antibody striped on to the reagent pad.

Any target toxin molecules found in the sample, bind to the color-labeled antibody.

Together the reagents flow up the device.

Any remaining unbound antibodies bind to the target toxin sprayed on the test line.

This forms a visible line.

The less intense the test line, the more toxin is present in the sample.

Excess color-labeled antibody binds to the anti-species antibody sprayed into the control line.

The control reaction occurs regardless of the presence or absence of toxin molecule.

This assures the user that the test worked correctly.

Neogen's lateral flow strip reader then calculates the quantitative results.

Negative Sample

The sample is added to the lateral flow device.

Liquid traveling up the device picks up the color-labeled antibody striped on to the reagent pad.

Since the sample contains no target tax and molecules, color-labeled antibody continues to flow up the device unbound.

Unbound antibodies bind to the target toxin sprayed on the test line.

This forms a visible line.

The more intense the test line the less toxin is present in the sample.

Excess color-label antibody binds to the anti species antibody sprayed into the control line.

The control reaction occurs regardless of the presence or absence of toxin molecule.

This assures the user that the test worked correctly.

Neogen's lateral flow strip reader then calculates the quantitative result.

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Category: Science Insights