Better Mycotoxin Testing Begins with Better Samples

April 04, 2024

As the seasons change and harvest times approach, the importance of mycotoxin testing in ensuring food and feed safety becomes increasingly evident. Mycotoxins, toxic compounds produced by fungi, pose significant risks to human and animal health, making accurate and efficient testing paramount. In this blog, we will explore some essential tips for mycotoxin testing, from sample collection to analysis, and highlight the value of Neogen's Mycotoxin Handbook as a comprehensive resource for industry professionals. Additionally, we will delve into the challenges associated with mycotoxin testing, including regulatory compliance, method accuracy, and the need for rapid results, underscoring the critical role of robust testing protocols in safeguarding public health and maintaining product quality. Understanding these challenges enables businesses to implement effective testing strategies, ensuring consumer safety and regulatory compliance while mitigating risks associated with mycotoxin contamination.

What are Mycotoxins?

Mycotoxins are secondary metabolites produced by fungi as they grow rapidly in specific environmental conditions. These toxins can contaminate various commodities, particularly grains, both before and after harvest. The growth of fungi and subsequent mycotoxin production depend on factors like temperature, humidity, and the availability of organic material.

Understanding Mycotoxin Risks, Detection, and Management

Mycotoxins pose significant health risks to humans and livestock. Aflatoxin, for example, is highly toxic and carcinogenic, causing liver damage and cancer in exposed individuals. Other mycotoxins like deoxynivalenol (DON), fumonisin, ochratoxin, and zearalenone have diverse effects on different species, ranging from gastrointestinal issues to reproductive problems and immune suppression.

Detecting mycotoxins in commodities is crucial for protecting human and animal health. Various testing methods, such as high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA), are used to identify and quantify mycotoxins in samples. Proper storage conditions, including temperature and humidity control, can also help prevent fungal growth and mycotoxin production.

Mycotoxin Testing Challenges

Mycotoxin testing presents several challenges for quality assurance managers in flour mills, food processors, and pet and animal feed manufacturers. Seasonal hires without prior lab experience may require extensive training, while lab managers and technicians need to ensure regulatory compliance, testing accuracy, and efficient workflow processes. One challenge is to ensure that all staff are properly trained and confident in the sampling and testing protocols. This underscores the need to choose testing methods that best fit with the workflow and testing needs of the laboratory. Moreover, the complexity of mycotoxin analysis, including the wide range of toxins and the variability in contamination levels across different grains and geographical regions, adds another layer of difficulty. Additionally, keeping abreast of evolving regulations and technological advancements in testing methodologies poses ongoing challenges for industry professionals. Addressing these multifaceted challenges requires a comprehensive approach that integrates training, regulatory compliance, and advancements in testing technology to ensure the safety and quality of food and feed products.

Best Practices for Mycotoxin Sampling

One of the critical aspects of mycotoxin testing is sample collection. You must obtain a representative sample from the grain load, grind a sufficient amount of the sample to a consistent particle size, and test the recommended portion size of the ground sample. There are many resources available to determine an optimal sample probing of the incoming grain to best represent the entire load.

Understanding the nuances of mycotoxin testing is crucial for ensuring the safety and quality of food and feed products. Key considerations include particle size for efficient extraction, the use of mycotoxin reference material (MRM) to validate methods, and following advisory and regulatory limits to ensure compliance. Another critical element in a mycotoxin program is a robust sampling plan. There are many resources available to guide you in efficient probing patterns, ensuring a representative sample from the grain load. The next step is grinding an adequate amount of the sample, then taking a sub-sample for testing. All these elements will be essential to obtaining a homogenous sample for your testing program.

Another best practice for your mycotoxin program is enrollment in a proficiency program. Using reference material will help determine additional training needs and provide documentation that your mycotoxin program is on track. There are many factors that can affect the quality of the results generated by testing including following protocols, obtaining homogenous samples, and using calibrated equipment. Understanding the limitations of the testing method chosen in your lab and using proper pipetting techniques can improve the quality of the results generated.

Interpreting Your Samples’ Mycotoxin Test Results

Once samples are collected, accurately interpreting the test results will be crucial for decision-making. Understanding the regulatory requirements for your region is essential in ensuring compliance. The use of MRM (Mycotoxin Reference Material) helps demonstrate the method chosen is appropriate for the range of testing needed. Proper interpretation of test results enables you to implement appropriate mitigation strategies and ensure food and feed safety.

Why Neogen's Mycotoxin Handbook Matters

Neogen's Mycotoxin Handbook serves as an invaluable resource for industry professionals seeking comprehensive guidance on mycotoxin testing. Covering everything from sample collection techniques to analytical methodologies, the handbook provides practical insights and best practices for ensuring the safety and quality of food and feed commodities. With contributions from Neogen's tech team, the handbook offers expert advice tailored to the specific challenges of mycotoxin testing.

Access the Neogen Mycotoxin Handbook Today

Ready to elevate your mycotoxin testing practices? Download Neogen's Mycotoxin Handbook today to access essential tips, guidelines, and industry insights. Whether you're a seasoned quality assurance manager or a lab technician navigating the complexities of mycotoxin analysis, this handbook equips you with the knowledge and tools needed to ensure better testing outcomes.

Get the facts with the Neogen Mycotoxin Handbook and embark on a journey towards safer food and feed production.


Category: Agriculture, Milling & Grain, Mycotoxins