Megazyme® Lichenase (endo-1,3:1,4-β-D-Glucanase) (Bacillus subtilis)

5,000 Units

SKU No.  700004227  |  Catalog No.  E-LICHN

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

High purity Lichenase (endo-1,3,1,4-β-Glucanase) (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications.

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Specifications
BrandMegazyme®
EC Number

3.2.1.73

Enzymeβ-Glucanase, Lichenase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food and feeds, brewing and biofuels industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number

37288-51-0

CAZy FamilyGH16
Concentration~ 1000
Synonyms

licheninase; (1→3)-(1→4)-beta-D-glucan 4-glucanohydrolase

SourceBacillus subtilis
Molecular Weight

26750

ExpressionPurified from Bacillus subtilis
Specificity

Hydrolysis of (1,4)-β-D-glucosidic linkages in β-D-glucans containing (1,3)- and (1,4)-bonds.

Specific Activity

~ 230 U/mg (40°C, pH 6.5 on barley β-glucan)

Unit Definition

One Unit of lichenase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from barley β-glucan (10 mg/mL) in sodium phosphate buffer (100mM), pH 6.5 at 40°C.

Temperature Optima (°C)60
pH Optima6
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.04 lb